Global analysis of symbiotic tissue specific gene expressions of nitrogen and carbon metabolisms in legume nodules

1)对大豆根瘤线粒体和根瘤类杆菌的蛋白质组分析进行了综述。蛋白质质量指纹图谱和LCMMS分析表明，465个斑点(根部线粒体)和383个斑点(根部线粒体)具有组织特异性。利用Edman降解法、蛋白质质量指纹图谱和LCMSMS分析对这些蛋白质斑点进行鉴定。虽然有许多功能没有在数据库中注册的斑点，但数据显示有7个上调蛋白和8个下调蛋白被鉴定出来。在结节线粒体中也发现了6种受抑制的蛋白质。对这些蛋白在根瘤代谢中的作用进行了讨论。对结节类杆菌也采用了类似的办法。在这项类菌蛋白质组实验中，我们确定了62种蛋白质在根瘤类杆菌中比培养细菌上调。2)为了准备基因组测序数据，我们加入了Kazusa DNA研究所和Kouchi博士的实验室的项目组。在本实验中，我们从克隆的5‘端对9000多个克隆进行了测序，数据将通过Kazusa DNA研究所提供给公众。

1)Proteome analysis of nodule mitochondria and nodule bacteroids of soybean plants was surveyed, In. the case of nodule mitochondria 2D-EP following Protein Mass Finger print and LCMSMS analysis revealed that 465 spots (nodule mitochondria) and 383 spots (root mitochondria) were tissue specific. Using Edman degradation methods, Protein Mass Finger print and LCMSMS analysis identification of these protein spots were performed. Although there are many spots which the function were not registered in the database, the data showed 7 up-regulating and 8 down-regulating proteins were identified. Six suppressed proteins in nodule mitochondria were identified too. The role in nodule metabolism of these proteins were discussed. Similar approach was employed in the case of nodule bacteroids too. In this bacteroid proteome experiment, we identified 62 proteins which were up-regulated in nodule bacteroids comparing to cultured bacteria.2)For preparing genome sequencing data we joined to the project team of Kazusa DNA Research Institute and Dr. Kouchi' laboratory. In this experiment we sequenced more than 9,000 cDNA clones from 5'site of the clone and the data is going to supplied to the public through Kazusa DNA Research Institute.