Functional analysis of non-coding RNA in production of extracellular toxin in virulent bacteria.

强毒细菌胞外毒素产生过程中非编码 RNA 的功能分析。

• 批准号: 14560057
• 负责人: NAKAMURA Kouji
• 金额: $ 2.3万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560057/
• 关键词: BS190 RNA; noncoding RNA; RNA world; Clostridium perfringens; riboregulation; regulatory RNA; gene regulation; virulence; 病原細菌; VirR; VirS; 2成分制御系; 毒素産生; VR-RNA; 転写制御; 二成分制御系; 5'非翻訳領域

A novel RNA species was isolated from Bacillus subtilis, and its sequence was determined and mapped to its genetic position. This RNA was termed BS190 RNA from the length of its mature form (190nt), and the gene encoding it is located within the aspS-yrvM intergenic region of the B.subtilis genome. A computer-aided prediction of the secondary structure of BS190 RNA showed that it can be folded into a single hairpin structure with some bulge structures. We found that the growth rate of a ΔBS190 mutant strain of B.subtilis was reduced when compared to the wild-type. On the other hand, a virulent bacteria, clostridium perfringens, contains a novel noncoding RNA (VR-RNA) that is concerns with production toxin. Our data suggested that C.perfringens harbors at least two VR-RNA binding proteins and interacting with these proteins, VR-RNA regulates the expression of toxin gene at transcriptional level.

从枯草芽孢杆菌中分离出一种新的 RNA 物种，确定了其序列并绘制了其遗传位置。该 RNA 根据其成熟形式的长度 (190nt) 被称为 BS190 RNA，编码它的基因位于枯草芽孢杆菌基因组的 aspS-yrvM 基因间区域内。计算机辅助预测 BS190 RNA 的二级结构表明，它可以折叠成具有一些凸起结构的单个发夹结构。我们发现，与野生型相比，枯草芽孢杆菌 ΔBS190 突变株的生长速率降低。另一方面，产气荚膜梭菌这种剧毒细菌含有一种与产生毒素有关的新型非编码RNA (VR-RNA)。我们的数据表明，产气荚膜梭菌至少含有两个VR-RNA结合蛋白，并与这些蛋白相互作用，VR-RNA在转录水平上调节毒素基因的表达。

项目成果

Sakamoto, T., Nakamura, K.他: "Solution structure of a SRP19 binding domain in human SRP RNA."J.of Biochem.(Tokyo). 132. 177-182 (2002)

Sakamoto, T., Nakamura, K. 等人：“人 SRP RNA 中 SRP19 结合结构域的溶液结构。”J.of Biochem.（东京）132. 177-182 (2002)。

Suzuma, S., Nakamura, K.他: "Identification and characterization of novel small RNA in the aspS-yrvM intergenic region of the Bacillus subtilis genome"Microbiology. 148・8. 2591-2598 (2002)

Suzuma, S., Nakamura, K. 等人：“枯草芽孢杆菌基因组的 aspS-yrvM 基因间区域中新型小 RNA 的鉴定和表征”微生物学 148·8 (2002)。

Kumano, M., Nakamura, K.他: "Lincomycin resistance mutations in the two regions immediately downstream of the -10 region of 1mr promoter cause overexpression of a putative multidrug efflux pump in Bacillus subtilis mutants"Antimicrob.Agents Chemother.. 47・

Kumano, M., Nakamura, K. 等人：“紧邻 1mr 启动子 -10 区域下游的两个区域中的林可霉素抗性突变导致枯草芽孢杆菌突变体中推定的多药外排泵的过度表达”Antimicrob.Agents Chemother.. 47.

Suzuma, S., Nakamura, K., 他: "Identification and characterization of novel small RNAs in the asps-yrvM intergenic region of Bacillus subtilis."Microbiology. 148. 2591-2598 (2002)

Suzuma, S., Nakamura, K. 等人：“枯草芽孢杆菌 asps-yrvM 基因间区域中新型小 RNA 的鉴定和表征。”微生物学 148. 2591-2598 (2002)

Ando, Y.: "Expression of a small RNA, BS203 RNA, from the yocI-yocJ intergenic region of Bacillus subtilis genome"FEMS Microbiology Letters. 207・1. 29-33 (2002)

Ando, Y.：“来自枯草芽孢杆菌基因组的 yocI-yocJ 基因间区域的小 RNA BS203 RNA 的表达”FEMS 微生物学快报 207・1（2002）。