Functional analysis of non-coding RNA in production of extracellular toxin in virulent bacteria.

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A novel RNA species was isolated from Bacillus subtilis, and its sequence was determined and mapped to its genetic position. This RNA was termed BS190 RNA from the length of its mature form (190nt), and the gene encoding it is located within the aspS-yrvM intergenic region of the B.subtilis genome. A computer-aided prediction of the secondary structure of BS190 RNA showed that it can be folded into a single hairpin structure with some bulge structures. We found that the growth rate of a ΔBS190 mutant strain of B.subtilis was reduced when compared to the wild-type. On the other hand, a virulent bacteria, clostridium perfringens, contains a novel noncoding RNA (VR-RNA) that is concerns with production toxin. Our data suggested that C.perfringens harbors at least two VR-RNA binding proteins and interacting with these proteins, VR-RNA regulates the expression of toxin gene at transcriptional level.