Study on structure and function of thiamin-binding protein and its utilization for improvement of seed

硫胺素结合蛋白的结构、功能研究及其在种子改良中的应用

• 批准号: 14560086
• 负责人: WATANABE Katsumi
• 金额: $ 2.3万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560086/
• 关键词: Seed storage protein; Thiamin-binding protein; Thiamin; Sesame; Sesame seed; Wheat; Wheat seed; 植物種子

The thiamin-binding activity of sesame and wheat seeds increased with the seed development after flowering, and then decreased during seed germination. The immunological analysis using the specific antibody against the thiamin-binding protein showed that the change of the protein was similar to that of the binding-activity in those seeds and that the protein was accumulated only in the peripheral cells such as albumen cells and aleurone layer. Moreover, the expression of the gene encoding thiamin-binding protein was found only in seeds during maturation in the case of sesame. On the other hand, the thiamin content of the seeds increased remarkably during maturation, and then decreased during germination. These results suggested that thiamin was accumulated and stored as a complex with the protein in mature seeds.

芝麻和小麦种子的硫胺素结合活性在开花后随种子发育而增加，然后在种子萌发过程中下降。用硫胺素结合蛋白的特异性抗体进行免疫学分析表明，该蛋白的变化与种子中结合活性的变化相似，仅在蛋白细胞和糊粉层等外围细胞中积累。此外，编码硫胺素结合蛋白的基因只在芝麻成熟过程中的种子中表达。另一方面，种子中硫胺素含量在成熟过程中显著增加，在萌发过程中呈下降趋势。这些结果表明，硫胺素在成熟种子中以与蛋白质的络合物的形式积累和储存。

项目成果

Katsumi Watanabe: "Synthesis of thiamin-binding protein during seed development and its accumulation in sesame seed"Plant Biotechnology. 20(3). 221-224 (2003)

Katsumi Watanabe：“种子发育过程中硫胺素结合蛋白的合成及其在芝麻中的积累”植物生物技术。

Katsumi Watanabe, Naoko Nishida, Takashi Adachi, Motoko Ueda, Toshuo Mitsunaga, Yukio Kawamura: "Accumulation and degradation of thiamin-binding protein and level of thiamin in wheat seeds during seed maturation and germination."Bioscience Biotechnology B

Katsumi Watanabe、Naoko Nishida、Takashi Adachi、Motoko Ueda、Toshuo Mitsunaga、Yukio Kawamura：“种子成熟和发芽期间小麦种子中硫胺素结合蛋白的积累和降解以及硫胺素水平。”Bioscience Biotechnology B

Katsumi Watanabe: "Change of thiamin-binding protein and thiamin levels during seed maturation and germination in sesame"Plant Physiology Biochemistry. 41(11). 973-976 (2003)

Katsumi Watanabe：“芝麻种子成熟和发芽过程中硫胺素结合蛋白和硫胺素水平的变化”植物生理学生物化学。

Katsumi Watanabe: "Accumulation and degradation of thiamin-binding protein and level of thiamin in wheat seeds during seed maturation and germination"Bioscience Biotechnology Biochemistry. In press. (2004)

Katsumi Watanabe：“种子成熟和发芽过程中小麦种子中硫胺素结合蛋白的积累和降解以及硫胺素水平”生物科学生物技术生物化学。

Katsumi Watanabe: "Change of thiamin-binding protein and thiamin levels during seed maturation and gemination in sesame"Plant Physiology and Biochemistry. 41. 973-976 (2003)

Katsumi Watanabe：“芝麻种子成熟和发芽过程中硫胺素结合蛋白和硫胺素水平的变化”植物生理学和生物化学。