Study on structure and function of thiamin-binding protein and its utilization for improvement of seed

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The thiamin-binding activity of sesame and wheat seeds increased with the seed development after flowering, and then decreased during seed germination. The immunological analysis using the specific antibody against the thiamin-binding protein showed that the change of the protein was similar to that of the binding-activity in those seeds and that the protein was accumulated only in the peripheral cells such as albumen cells and aleurone layer. Moreover, the expression of the gene encoding thiamin-binding protein was found only in seeds during maturation in the case of sesame. On the other hand, the thiamin content of the seeds increased remarkably during maturation, and then decreased during germination. These results suggested that thiamin was accumulated and stored as a complex with the protein in mature seeds.