Studies on the development of fucoidan prepared from commercially cultured Cladosiphon okamuranus TOKIDA(Okinawamozuku)

我之前从商业养殖的Cladosiphon okamuranus TOKIDA（Okinawamozuku）中分离出一种岩藻聚糖，其产量估计为2.0-2.3%（湿海藻）。岩藻多糖具有降胆固醇、抗凝血、抗肿瘤等生理和生物活性。我在此报告从商业养殖的冲绳岛（中国村）南部海域的渔网上制备的岩藻糖胶的流变学性质和血液抗凝血活性。岩藻糖聚糖由L-焦糖：d -木糖：d -葡萄糖醛酸：乙酸：硫酸=4.0:0.1:1.0:2.0:2.0组成。岩藻聚糖的分子量估计为500000。采用同轴圆柱体和旋转外圆柱体的流变仪测量了岩藻糖聚糖的非牛顿行为和动态粘弹性。岩藻聚糖在25℃时的流动曲线在1.5%以下表现为剪切减薄行为，在2.0%时表现为塑性行为。在不同浓度的褐藻聚糖溶液中，动态粘度略高于存储模量。NaCl（0.1%）和CaCl_2（0.1%）加入到2.0%岩藻糖聚糖溶液中，动态粘弹性增加。岩藻聚糖的动态粘弹性在较宽的pH范围内是稳定的。褐藻聚糖在水溶液中具有独特的神学特性，可用于食品、化妆品等行业。用人血浆对岩藻聚糖进行了血液抗凝活性试验，并与肝素进行了比较。凝血酶和活化凝血活素活性高于肝素。硫酸和甲酸部分水解的褐藻多糖活性高于天然褐藻多糖。

I have previously isolated a fucoidan from commercially cultured Cladosiphon okamuranus TOKIDA(Okinawamozuku), the yield of which was estimated to be 2.0-2.3%(wet seaweed). Fucoidan has physiological and biological activities of an cholesterol lowering, anti-coagulant and anti-tumor agent. I report herein rheological properties and blood anti-coagulant activity of the fucoidan prepared from commercially cultured Okinawamozuku on nets at sea off the southern part of Okinawa Island(Chinen Village),Japan.The fucoidan consisted of L-fucose:D-xylose:D-glucuronic acid:acetic acid:sulfuric acid=4.0:0.1:1.0:2.0:2.0. The molecular weight of the fucoidan was estimated to be 500,000.The non-Newtonian behavior and dynamic viscoelasticity of the fucoidan were measured with a rheogoniometer equipped with a coaxial cylinder and rotating outer cylinder. The flow curves of the fucoidan, at 25℃, showed shear-thinning behavior below 1.5%, but plastic behavior at 2.0%. A little higher dynamic viscosity was observed than that of storage modulus in fucoidan solutions at various concentrations. The dynamic viscoelasticity increased with addition of NaCl(0.1%) and CaCl_2(0.1%) to 2.0% fucoidan solution. The dynamic viscoelasticity of the fucoidan was stable over a wide pH range. The fucoidan had unique Theological characteristics in aqueous solution, therefore it migh be able to use in foods, cosmetics and some other industries.Blood anti-coagulant activity tests were conducted on th fucoidan with human plasma in comparison with heparin. The thrombin and activated thromboplastin activity was higher than that of heparin. Partially hydrolyzed fucoidans with sulfuric acid and formic acid had higher activity that that of native fucoidan.