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Nutritional assessment of artificial microdiets for larval fish and crustaceans

幼鱼和甲壳类动物人工微量饲料的营养评估

基本信息

批准号：
14560172
负责人：
TESHIMA Shinichi
金额：
$ 2.24万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560172/
关键词：
Laraval fish Microparticulate diet Red sea bream Japanese flounder Nutritional assessment Sinking speed Diet 人工微粒子飼料栄養学的評価給餌

项目摘要

In order to enhance the development of artificial microdiet(MD), MD were prepared and assessed nutritionally by using the larval red sea bream Pagrus major and Japanese flounder Paralichthys olivaceus as test fish.First, nitrogen and lipid sources were searched and investigated in order to make a high quality MD which is stable in sea water and contains nutritionally well-balanced nutrients.As a result, MD containing soybean peptides and fish oil calcium soap(FOCS)as the major nitrogen and lipid sources and zein as a binder(abbreviated as MBD)were found to fit to the purpose of the present study.First feeding experiment (study A)were conducted using red sea bream(30 days old)and Japanese flounder(15 days old)with several MBD containing soybean peptides with different, molecular weights, soybean protein isolate, FOCS, stearoyl calcium lactate, etc.It was possible to rear both the fish without using any live food such as rotifer Brachionus plicatilis and Artemia nauplii.Soybean peptides … More with molecular weights of about 1000 Da and FOCS had a high dietary value as nitrogen and lipid sources for both the fish.In the next feeding experiment(study B), feeding experiments similar to those in study A were conducted using the first feeding(5 days old)red sea bream and Japanese flounder.Both the larval fish were successfully reared by feeding the MBD alone during the daytimes if small amounts(1/10 of the live food, control)of the rotifer were provided only in the evening to prevent the starvation during the nighttime.Finally, study C was conducted to clarify the effects of diet composition and feeding methods on the sinking speed and intake of diet and performance of the red sea bream and Japanese flounder.The sinking speed varied with diet formulation, and the diet intake by the both fish(15 days old)changed with the sinking speed of diet and feeding frequency.Also, growth and survival of the flounder(5 days old)were altered with sinking speeds of diet provided.From the results of the present study, it is recommended to assess the physico-chemical properties of diet besides nutrient contents for the development of quality MD. Less

为促进人工微饲料（MD）的发展，以真鲷幼体和牙鲆幼体为试验鱼，制备MD并进行营养评价。含有大豆肽和鱼油钙皂（FOCS）作为主要氮和脂源，玉米蛋白作为粘合剂的饲料（简称MBD）被发现符合本研究的目的。第一次饲养实验（研究A）是用红海鲷（30日龄）和日本比目鱼（15日龄）和几种含有不同分子的大豆肽的MBD进行的。重量，大豆分离蛋白，FOCS，硬脂酰乳酸钙等。可以在不使用任何活饵料（如褶皱臂尾轮虫和卤虫无节幼体）的情况下饲养这两种鱼。大豆肽...更多分子量约为1000 Da，FOCS作为两种鱼的氮和脂源具有很高的膳食价值。在接下来的饲养实验中（研究 B），使用第一次喂养（5天龄）红海鲷和日本比目鱼进行了与研究A类似的喂养实验。如果仅在晚上提供少量（1/10的活食，对照）轮虫以防止夜间饥饿，则两种幼鱼均通过白天单独喂养MBD成功饲养。饲喂方法对红鲷鱼和牙鲆的下沉速度、饲料摄入量和生产性能的影响。下沉速度随饲料配方的不同而变化，两种鱼（15日龄）的饲料摄入量随饲料下沉速度和投喂频率的变化而变化。而且，比目鱼（5日龄）的生长和存活率也随所提供饲料的下沉速度而改变。从本研究的结果来看，建议对鱼的理化指标进行评估。除了营养成分外，饮食的特性也有助于发展优质MD。较少的