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Transportation selection mechanism between nucleus and cytoplasm in the nuclear pore complex Approach from morphology

核孔复合体中细胞核与细胞质之间的运输选择机制从形态学角度研究

基本信息

批准号：
14570012
负责人：
NAGURO Tomonori
金额：
$ 1.86万
依托单位：
National University Corporation Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570012/
关键词：
nuclear membrane nuclear pore complex fine structure SEM nuclear pore three-dimensional structure

项目摘要

All eight kinds of surfaces of nuclear membranes (four true surfaces of the outer and inner nuclear membranes, four fracture faces of the outer and inner nuclear membranes) in the rat pancreatic acinar cell were exposed by adjusting the density and the kind of fixatives and/or by controlling the density of antifreeze solution at the freeze cracking process. The exposed surfaces were ion-coated with osmium and they were investigated with high-resolution scanning electron microscope(SEM).As the proteins consisting nuclear pore complex(NPC) were tend to disappear at the maceration treatment process with 0.1% osmium solution, the observation of the nuclear pore without proteins of NPC about 45-65 nm in diameter could be possible both from the cytoplasm side and nucleus side. The membrane surrounding the nuclear pores rose slightly like the bank, and it seemed to form a ring of about 90 nm in diameter on the true surface of inner nuclear membrane.SEM observation from the cytoplasm side reve … More aled that eight particles consisting the cytoplasmic part of NPC were not spherical but ovoid in shape. Since these particles disappeared rather easily at the maceration treatment process, it became possible to observe the true surface of outer nuclear membrane without the particles. The surface was smooth and flat appearance, and so it seemed to remain intact. This result indicates that the particles are situated on the true surface of outer nuclear membrane having little connection with the membrane.The size of transporting material ("central plug" or "cargo" or "cargo and transport factors") was about 25-30 nm in diameter. The central part of NPC surrounding the transporting materiel formed a central ring of about 10-35 nm in inner diameter, 45-65 nm in outer diameter. It was composed of sixteen particles. The ring like structure of about 45-65 nm in inner diameter and about 90-100 nm in outer diameter situated in the perinuclear space was also composed of sixteen particles. The both composition protein particles in the perinuclear space and in the center of nuclear pore seemed to form a ring connecting tightly to each other across the membrane probably in the form of dimmer units. Less

通过调节冷冻裂解过程中固定剂的种类和/或防冻液的浓度，暴露了大鼠胰腺腺泡细胞核膜的全部8种表面（外核膜和内核膜的4个真实面，外核膜和内核膜的4个破裂面）。暴露的表面用锇离子包被，用高分辨扫描电镜（SEM）观察，由于组成核孔复合物（NPC）的蛋白质在0.1%锇溶液浸渍处理过程中趋于消失，因此可以从细胞质和细胞核两个侧面观察到直径约45-65 nm的无NPC蛋白质的核孔。核孔周围的膜呈堤岸状微隆起，在核膜的真表面上形成一个直径约90 nm的环，从细胞质侧扫描电镜观察，核孔周围的膜呈圆形，核孔周围的膜呈圆形，核孔周围的膜 ...更多信息结果表明，NPC细胞质部分的8个颗粒不是球形，而是卵圆形。由于这些颗粒在浸渍处理过程中很容易消失，因此可以观察到没有颗粒的外核膜的真实表面。表面光滑平整，看起来完好无损。这一结果表明，这些颗粒位于外核膜的真实表面，与核膜几乎没有联系。转运物质（“中心塞”或“货物”或“货物和转运因子”）的直径约为25-30 nm。NPC的中心部分围绕着运输物质形成一个内径约10-35 nm、外径约45-65 nm的中心环。它由16个粒子组成。位于核周空间的环状结构也由16个颗粒组成，内径约45-65 nm，外径约90-100 nm。核周间隙和核孔中心的两种蛋白质颗粒似乎形成了一个环，它们可能以二聚体的形式穿过膜彼此紧密相连。少