权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of carriage and transmission of Clostridium difficile

艰难梭菌的携带和传播分析

基本信息

批准号：
14570233
负责人：
NAKAMURA Shinichi
金额：
$ 2.56万
依托单位：
KANAZAWA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

In addition of the last year, another one group of healthy individuals(n=33,age range,20-29) was examined for colonization of Clostridium difficile a total of 3 times at intervals of 3 months. Isolates were analyzed by PCR ribotyping and pulsed-field gel electrophoresis(PFGE) to investigate the colonization status of C.difficile. Moreover, intestinal microflora of C.difficile-positive and -negative subjects was compared. 1.Colonization of C.difficile : C.difficile was isolated from nine(9.1%) of 99 samples. Colonization rates were 9.1% at all three examinations and were similar to those in previous study. Among the four positive subjects, the number of subjects from whom C.difficile was isolated once, twice, three times was two, one and one, respectively. 2.Colonization status of C.difficile : All nine isolates form four specimens were analyzed by PCR ribotyping and PFGE. C.difficile of the same PCR ribotype/PFGE type of were isolated from three subjects. The PCR ribotype/PFGE type of the isolated from a subject at the another examination was changing. 3.Comparison of intestinal micro flora : Composition of feacal micro flora was compared in C.difficile-positive(n=12) and -negative(n=12) subjects. C.difficile-positive subjects were significantly more densely colonized by enterococci than C.difficile-negative subjects. These results suggest that colonization of healthy individuals by C.difficile・is transient in many cases, however, indicates that there are healthy individuals who were continuously colonized by C.difficile. Moreover, these data suggest that dense colonization of the intestine by enterococci may be associated with C.difficile colonization.

除去年外，对另一组健康个体（n=33，年龄范围，20-29）进行了艰难梭菌定殖检查，共3次，间隔3个月。分离株进行了分析，通过PCR核糖分型和脉冲场凝胶电泳（PFGE）调查艰难梭菌的定植状态。此外，还比较了艰难梭菌阳性和阴性受试者的肠道菌群。1.艰难梭菌的定植：从99份样本中的9份（9.1%）中分离出艰难梭菌。所有三次检查的定植率均为9.1%，与既往研究相似。在4例阳性受试者中，分离出艰难梭菌1次、2次、3次的受试者数量分别为2例、1例和1例。2.艰难梭菌的定殖状态：通过PCR核糖分型和PFGE分析了来自4份标本的所有9种分离株。从三名受试者中分离出相同PCR核糖体型/PFGE型的艰难梭菌。在另一次检查中从受试者分离的PCR核糖体型/PFGE型发生变化。3.肠道微生物植物群的比较：在艰难梭菌阳性（n=12）和阴性（n=12）受试者中比较粪便微生物植物群的组成。艰难梭菌阳性受试者的肠球菌定植密度显著高于艰难梭菌阴性受试者。这些结果表明，艰难梭菌在健康个体中的定殖在许多情况下是短暂的，然而，表明存在被艰难梭菌持续定殖的健康个体。此外，这些数据表明肠球菌在肠道的密集定植可能与艰难梭菌定植有关。