CYP2A6 and CYP2E1 immunoreactivities in bronchial epithelial cells affected by smoking in non-small cell lung cancer patients.

细胞色素P450（CYP）2A 6和CYP 2 E1是众所周知的激活前致癌物的酶，如亚硝胺（烟草烟雾中的溶剂）和DNA加合物的形式。在支气管上皮细胞（BEG）中也观察到CYP 2A 6和CYP 2 E1 mRNA。本研究检测了78例非小细胞肺癌（NSCLC）患者BEC中CYP 2A 6和CYP 2 E1的免疫反应性及肿瘤中p53的免疫反应性。我们研究了CYP 2A 6和CYP 2 E1在BEC中的表达与终生吸烟量（LTCC）的关系。我们还揭示了CYP 2A 6和CYP 2 E1在BEC和LTCC中表达阳性数之间的关系。[材料与方法]我们检测了78例日本NSCLC患者的CYP 2A 6、CYP 2 E1、p53免疫反应性，这些患者在获得适当的知情同意后在UOEH接受手术切除。根据WHO分类进行肿瘤的组织学分型（腺癌; 48例，鳞状细胞癌; 30例）。不 关于我们 根据Brinkman指数（BI ;烟支/天x年）分类的LTCC量。通过LSAB（标记的链霉亲和素生物素）方法进行免疫组织化学染色（IHC）。当超过10%的BEC被染色时，确定CYP 2A 6和CYP 2 E1表达为阳性，当超过10%的肿瘤细胞被染色时，确定p53表达为阳性。[结果与结论] 78例非小细胞肺癌中CYP 2A 6和CYP 2 E1的阳性率分别为53%（41/78）和22%（17/78）。p53阳性率为53%（41/78）。30例鳞癌的CYP 2A 6阳性率（60%）高于48例腺癌（48%）。30例鳞癌p53蛋白阳性率（57%）明显高于48例腺癌（50%）。CYP 2 E1表达与LTCC有显著相关性（p<0.05）。RT-PCR阳性表达数与LTCC的发生有显著相关性（p<0.05）。CYP 2A 6和CYP 2 E1在NSCLC I期患者中的表达与CYP 2A 6和CYP 2 E1阴性患者相比，生存期缩短，研究CYP 2A 6和CYP 2 E1在BEC中的表达，不仅有助于准确预测肿瘤发生的风险，而且有助于制定个体化的肿瘤预防方案。少

[Introduction] Cytochrome P450 (CYP) 2A6 and CYP2E1 are well-known enzymes that activate procarcinogens, such as the nitrosamines (solvents in tobacco smoke) and that form of DNA adduct. CYP2A6 and CYP2E1 mRNA are also observed in bronchial epithelial cells (BEG). In this study, CYP2A6 and CYP2E1 immunoreactivities in BEC and p53 immunoreactivities in tumor were examined in 78 non-small cell lung cancer (NSCLC) patients. We investigated the relationships between CYP2A6 and CYP2E1 expression in BEC and lifetime cigarette consumption (LTCC). We also revealed the relationships between positive numbers of CYP2A6 and CYP2E1 expression in BEC and LTCC.[Materials and Methods] We examined CYP2A6 CYP2E1, p53 immunoreactivities in 78 Japanese patients with NSCLC who underwent surgical resection at UOEH after obtaining appropriate informed consent. Histological typing of the tumors was performed according to the WHO classification (adenocarcinoma ; 48 cases, squamous cell carcinoma ; 30 cases). T … More he amount of LTCC classified by the Brinkman Index (BI ; cigarettes/day x years). Immunohistochemical staining (IHC) was performed by the LSAB (labelled streptavidine biotin) method. CYP2A6 and CYP2E1 expression was determined as positive when more than 10% of BEC were stained and p53 expression as positive when more than 10% of tumor cells were stained.[Results and conclusions] The CYP2A6 and CYP2E1 positive rates in 78 NSCLC were 53% (41/78) and 22% (17/78), respectively. The p53 positive rate was 53% (41/78). The CYP2A6 positive rate (60%) in 30 squamous cell carcinoma seemed to be higher than that (48%) in 48 adenocarcinoma. The p53 positive rate (57%) in 30 squamous cell carcinoma also seemed to be higher than that (50%) in 48 adenocarcinoma. There was a significant relation between CYP2E1 expression and LTCC (p<0.05). There was also a significant relation between positive numbers of CYP expression and LTCC (p<0.05). The CYP2A6 and CYP2E1 positive cases seemed to have shortened survival compared with the CYP2A6 and CYP2E1 negative cases in NSCLC patients with stage I disease.To study CYP2A6 and CYP2E1 expression in BEC, it may be useful not only accurately to predict the risk of cancer development, but also to formulate individually-tailored plans for cancer prevention. Less