Relationship between CC chemokine expression in the airways of severe asthmatics and steroid resistance

在所研究的哮喘严重程度的生物标志物中，血浆嗜酸粒细胞趋化素水平不受皮质类固醇治疗的显著影响，即使在类固醇治疗存在的情况下，也与哮喘缓解期患者的严重程度有关。不稳定型哮喘患者治疗前血浆嗜酸粒细胞趋化因子水平与激素治疗后功能改善程度显著相关。这些结果表明，血浆嗜酸粒细胞趋化蛋白水平是不稳定哮喘患者疾病活动性和/或激素治疗反应的标志。哮喘患者呼出的呼吸冷凝液中可检测到缓激肽浓度，但与正常人无差异。缓激肽对人胎儿成纤维细胞(HFL-1)嗜酸性粒细胞趋化因子的产生有轻微的促进作用。肿瘤生长抑素-M或转化生长因子-α-β1对该细胞有较强的促嗜酸性粒细胞趋化作用，与转化生长因子-β-1共同作用可进一步增强类固醇对嗜酸性粒细胞趋化因子的抑制作用。

Among biomarkers of asthma severity studied, plasma eotaxin level was not significantly affected by corticosteroid treatment, and was associated with the severity of stable asthmatics even in the presence of steroid therapy. There was a significant correlation between the pretreatment levels of plasma eotaxin and the extent of functional improvement after steroid treatment in unstable asthmatics. These results suggest that plasma eotaxin level is a marker of disease activity and/or treatment response to steroids in unstable asthma.Bradykinin concentrations in exhaled breath condensate in asthmatics were detectable but were not different from those in normal subjects. Eotaxin production in human fetal fibroblast (HFL-1) was slightly increased by Bradykinin. TNF-α strongly induced eotaxin production in this cell line which was moderately inhibited by the addition of oncostatin-M or TGF-β1. The inhibitory effects of steroid on eotaxin production were further enhanced by coincubation with TGF-β1.