Correlations of gene polymorphism of interferon-induced proteins

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Single nucleotide polymorphism (SNP) in promoter region of MxA gene was compared between 29 asymptomatic carriers of hepatitis C virus (ASC) and 60 patients with chronic hepatitis C (CH). All patients gave written informed consents. SNP of MxA gene was determined by polymerase chain reaction-based restriction fragment length polymorphism (RFLP) according to the report of Hijikata et al. DNA was extracted from 200μ1 of whole blood. DNA was subjected to PCR for amplifying a 599-nt DNA fragment that covers the promoter region of MxA (nt-567-nt+30). Products of PCR were digested with HhaI and electrophoresed in 5% polyacrylamide gel.G/T heterozygote at nt-88 of promoter of MxA gene was significantly more frequently detected in ASC (66%) than in CH (28%)(p=0.008). G/G homozygote was significantly less frequently detected in ASC (17%) than in CH (52%)(p=0.019). T allele frequency was significantly higher in ASC (50%) than in CH (34%)(p=0.042).Carriers of T at nt-88 express MxA protein more efficiently than those of G when given interferon. Thus the carriers of T could easily clear hepatitis C virus by MxA protein in the early stage of infection, when hepatitis occurs and intrinsic interferon is released. This may be the reason why T allele frequency was significantly higher in ASC than in CH.