Transcriptional regulation of transcription factor Cdx2 in intestinal mucosa

肠粘膜转录因子Cdx2的转录调控

• 批准号: 14570501
• 负责人: MUTOH Hiroyuki
• 金额: $ 2.5万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570501/
• 关键词: Cdx2; Intestinal metaplasia; Transgenic mouse; Absorptive enterocytes; PCFS; Math1; Enteroendocrine cells; Stroma; intestinal metaplasia

In the progression of chronic gastritis, gastric mucosal cells deviate from the normal pathway of gastric differentiation to an intestinal phenotype. Many epidemiological studies have found an association between the formation of intestinal metaplasia and the development of gastric carcinoma. However, there is no direct evidence which shows intestinal metaplasia is a precursor lesion of gastric carcinoma to date. We periodically examined the intestinal metaplastic mucosa of Cdx2-transgenic mice we have previously generated. Gastric polyps developed from intestinal metaplastic mucosa in all stomachs of Cdx2-transgenic mice examined. These gastric polyps consisted of intestinal-type adenocarcinoma that invaded the submucosa and muscularis propria, and occasionally spread into the subserosa. p53 and APC gene mutations were recognized in the adenocarcinomas. The participation of APC and p53 gene mutations in gastric carcinogenesis from the intestinal metaplasia was verified by the Cdx2-transgenic mice, carrying ApcMin mutation or p53 deficiency, that developed gastric polyps much earlier than Cdx2 alone. We successfully demonstrated that long-term intestinal metaplasia induces invasive gastric carcinoma. These results indicate that intestinal metaplasia itself plays a significant role in the genesis and progression of gastric carcinoma.

在慢性胃炎的发展过程中，胃粘膜细胞偏离胃分化的正常途径，向肠表型分化。许多流行病学研究发现肠上皮化生的形成与胃癌的发生有关。然而，迄今为止，还没有直接证据表明肠上皮化生是胃癌的前驱病变。我们定期检查我们以前产生的Cdx2转基因小鼠的肠粘膜化生。胃息肉的肠化生粘膜在所有的Cdx2转基因小鼠的胃检查。这些胃息肉包括浸润粘膜下层和固有肌层的膀胱型腺癌，偶尔扩散到浆膜下。p53和APC基因突变在腺癌中被识别。APC和p53基因突变参与肠上皮化生的胃癌发生，通过Cdx2转基因小鼠，携带ApcMin突变或p53缺陷，比单独Cdx2更早地发生胃息肉来验证。我们成功地证明了长期肠上皮化生诱导浸润性胃癌。这些结果表明肠化本身在胃癌的发生和发展中起着重要作用。

项目成果

Satoh K, Kawata H, Tokumaru K, Kumakura Y, Ishino Y, Kawakami S, Inoue K, Kojima T, Satoh Y, Mutoh H, Kihira K, Sugano K: "Change in apoptosis in the gastric surface epithelium And glands after eradication of Helicobacter pylori"Dig.Liver Dis.. 35. 78-84

Satoh K、Kawata H、Tokumaru K、Kumakura Y、Ishino Y、Kawakami S、Inoue K、Kojima T、Satoh Y、Mutoh H、Kihira K、Sugano K：“根除胃表面上皮细胞和腺体细胞凋亡的变化

Ratineau, C: "Cyclin D1 Represses the Basic Helix-Loop-Helix Transcription Factor, BETA2/NeuroD"J.Biol.Chem.. 277,11. 8847-8853 (2002)

Ratineau，C：“细胞周期蛋白 D1 抑制基本螺旋-环-螺旋转录因子，BETA2/NeuroD”J.Biol.Chem.. 277,11。

Ratineau C, Petry MW, Mutoh H, Leiter AB: "Cyclin D1 represses the basic helix loop helix transcription factor, BETA2/NeuroD"J.Biol.Chem.. 277. 8847-8853 (2002)

Ratineau C、Petry MW、Mutoh H、Leiter AB：“细胞周期蛋白 D1 抑制基本螺旋环螺旋转录因子 BETA2/NeuroD”J.Biol.Chem.. 277. 8847-8853 (2002)

Eda A, Osawa H, Satoh K, Yanaaka I, Kihira K, Ishino Y, Mutoh H, Sugano K: "Aberrant expression of CDX2 in Barrett's epithelium and inflammatory esophageal mucosa"J.Gastroenterol.. 38. 14-22 (2003)

Eda A、Osawa H、Satoh K、Yanaaka I、Kihira K、Ishino Y、Mutoh H、Sugano K：“Barrett 上皮和炎性食管粘膜中 CDX2 的异常表达”J.Gastroenterol.. 38. 14-22 (2003)

Mutoh H, Hakamata Y, Sato K, Eda A, Yanaka I, Honda S, Osawa H, Kaneko Y, Sugano K: "Conversion of gastric mucosa to intestinal metaplasia in Cdx2-expressing transgenic mice"Biochem.Biophys.Res.Commun.. 294. 470-479 (2002)

Mutoh H、Hakamata Y、Sato K、Eda A、Yanaka I、Honda S、Osawa H、Kaneko Y、Sugano K：“表达 Cdx2 的转基因小鼠中胃粘膜向肠化生的转化”Biochem.Biophys.Res.Commun。