Research for oxygen sensitive ionic channels of human vascular smooth muscle cells.

人血管平滑肌细胞氧敏感离子通道的研究。

• 批准号: 14570778
• 负责人: KATSUBE Yasuhiro
• 金额: $ 2.18万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570778/
• 关键词: ion channel; umbilical cord artery; vascular smooth muscle cell; oxygen sensitive; Ca channel; ATP dependent K channel; patch clamp

We had explored the oxygen sensitive ionic channels of human umbilical vascular smooth muscle cells using whole-cell voltage clamp method.We have found the following two major results in the experimental years (2002-2003).1.Ca channel of the VMS cells from umbilical artery was not modified by oxygen/hypoxia. We used the Ba^<2+> as a charge carrier because Ca^<2+> current was very small for evaluating the Ca channel.2.K channel was increased by changing the external solution from oxygenated to hypoxic solution. The increased currents were completely inhibited by glybenclamide indicating that the increased currents were ATP-sensitive K channel currents.These results were indicating that ATP-sensitive channel may play an important role for oxygen sensitivity of the human vascular smooth muscle cells.We will evaluate the contribution of the Na/Ca exchanger which also play an important role for oxygen-induced contraction of vasculature as a next project.

采用全细胞电压钳法对人脐血管平滑肌细胞的氧敏离子通道进行了研究。在实验年份（2002-2003）中，我们发现了以下两个主要结果。氧/缺氧对脐动脉VMS细胞Ca通道无影响。我们使用Ba^<2+>作为电荷载流子，因为Ca^<2+>电流对于评估Ca通道来说非常小。将外溶液由含氧溶液改为低氧溶液，增加了K通道。增加的电流被格列本脲完全抑制，表明增加的电流是atp敏感的K通道电流。这些结果提示atp敏感通道可能在人血管平滑肌细胞的氧敏感性中起重要作用。我们将在下一个项目中评估Na/Ca交换器的贡献，它在氧诱导的血管收缩中也起着重要作用。

项目成果

Jing Shen, et al.: "The role of endothelin in oxygen-induced contraction of the ductus arteriosus in rabbit and rat fetuses."Heart Vessels. 16. 181-188 (2002)

Jing Shen 等人：“内皮素在兔和大鼠胎儿氧诱导动脉导管收缩中的作用。”心脏血管。

Miharu Hajikano, et al.: "Diazoxide increases ATP-sensitive K^+ current of neonatal rat ventricular myocytes."Journal of Molecular and Cellular cardiology. 35. A31 (2003)

Miharu Hajikano 等人：“二氮嗪增加新生大鼠心室肌细胞的 ATP 敏感 Kk 电流。”分子与细胞心脏病学杂志。

勝部康弘, 他: "動脈管に存在する酸素感受性カリウムチャネルに関する分子生物学的研究"(財)日本心臓血圧研究振興会(平成12年度研究業績集). 1-5 (2001)

Yasuhiro Katsube 等人：“动脉导管中氧敏感性钾通道的分子生物学研究”日本心脏和血压研究基金会（2000 年研究成果）1-5（2001 年）。

Miharu Hajikano, Yasuhiro Katsube, Miki Watanabe, Ei Ikegami, Yoko Uchikoba, Mitsuhiro Kamisago, Ryuji Fukazawa, Schunichi Ogawa: "Diazoxide increases ATP-sensitive K^+ current of neonatal rat ventricular myocytes."J Mol Cell Cardiol. 35. A31 (2003)

Miharu Hajikano、Yasuhiro Katsube、Miki Watanabe、Ei Ikegami、Yoko Uchikoba、Mitsuhiro Kamisago、Ryuji Fukazawa、Schunichi Okawa：“二氮嗪可增加新生大鼠心室肌细胞的 ATP 敏感 K^ 电流。”J Mol Cell Cardiol。

勝部康弘: "酸素で閉じるカリウムチャネル"財)日本心臓血圧研究振興会(平成13年度研究業績集). 23-28 (2002)

胜部泰宏：“氧封闭钾通道”日本心脏和血压研究促进会（2001年研究成果）23-28（2002年）。