权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Genome expression profiling of heavy ion irradiation for head and neck cancer using in-house DNA chips.

使用内部 DNA 芯片对头颈癌重离子照射的基因组表达谱进行分析。

基本信息

批准号：
14570897
负责人：
MIZOE Jyunetsu
金额：
$ 2.24万
依托单位：
National Institute of Radiological Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Cultured cells of Ca9-22 (originating from squamous cell carcinoma of the gum), G361 (malignant melanoma of the skin), HSG (adenocarcinoma of the salivary gland) and HaCaT (keratinocyte of the skin) were irradiated with x-ray, carbon ions and neon ions. The post-irradiation cell survival rate for each cell lines was determined by the colony assay and a dead cell count taken by the dye exclusion method. The D_<10> and SF_4 show in the order from Ca9-22<G361<HSG<HaCaT in x-ray and G361<HSG<Ca9-22<HaCaT in carbon and neon ions. These findings confirmed that x-ray resistant G361 cells and HSG cells are well controlled by carbon and neon ions.Corresponding genes of x-ray and carbon ions irradiation were identified by gene expression analysis (gene chip U133A). The Ca9-22 cells were irradiated with x-ray and carbon ions, and gene expression analysis was performed four hours after irradiation. The number of up regulated genes was 144 after x-ray and 286 after carbon ions. The number of down regulated genes was 402 in x-ray and 331 in carbon ions.Similarly, G361 cells were irradiated with carbon ions. The number of up regulated genes was 85 and the number of down regulated genes was 111. Comparison of gene expression between Ca9-22 cells and G361 cells shows that 6 genes exhibited an up regulation commonly while six genes revealed a down regulation commonly after both irradiation. From the results of clinical research, malignant melanoma have a higher local control rate by carbon ion radiotherapy than squamous cell carcinoma. From investigations of the genes that are expressed in a specific or consistent manner in either of these cell lines, it was possible to estimate the group of carbon ion sensitive genes and the group of carbon ion resistant genes, respectively. This evidence suggests that certain already known gene groups such as CyclinD1, in particular, determine the differences in the effectiveness of heavy particle radiation.

用X射线、碳离子和霓离子对培养的Ca9-22(牙周鳞状细胞癌)、G361(皮肤恶性黑色素瘤)、HSG(涎腺癌)和HaCaT(皮肤角质形成细胞)细胞进行照射。通过集落形成试验和染料排斥法计数死亡细胞数，确定各细胞系照射后细胞存活率。D&lt；10&gt；和SF_4在X射线中按Ca9-22&lt；G361&lt；HSg&lt；HaCaT和G361&lt；HSg&lt；Ca9-22&lt；HaCaT在碳和霓离子中的顺序显示。这些结果证实了抗X射线的G361细胞和HSG细胞很好地受碳和霓离子的控制。通过基因表达分析(基因芯片U133A)鉴定了X射线和碳离子照射的相关基因。用X射线和碳离子照射Ca9-22细胞，4h后进行基因表达分析。X射线照射后上调基因数目为144个，碳离子作用后上调基因数目为286个。X射线和碳离子分别有402个和331个基因表达下调。同样，用碳离子照射G361细胞。上调基因为85个，下调基因为111个。比较Ca9-22细胞和G361细胞的基因表达，发现两种照射后6个基因普遍上调，6个基因普遍下调。从临床研究结果看，恶性黑色素瘤的碳离子放射治疗的局部控制率高于鳞癌。通过对在这两种细胞系中以特定或一致方式表达的基因的研究，可以分别估计碳离子敏感基因组和碳离子抗性基因组。这一证据表明，某些已知的基因组，如细胞周期蛋白D_1，尤其决定了重粒子辐射效果的差异。

项目成果

期刊论文数量（16）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Dose escalation study of carbon ion radiotherapy for locally advanced head-and-neck cancer

DOI：
10.1016/j.ijrobp.2004.02.067
发表时间：
2004-10-01
期刊：
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
影响因子：
7
作者：
Mizoe, J;Tsujii, H;Konno, A
通讯作者：
Konno, A

溝江純悦, 山本信治, 神津由直, 肥後盛洋, 加藤真樹, 関直彦, 瀧口正樹: "口腔扁平上皮癌由来細胞株を用いた放射線および重粒子線照射による遺伝子発現プロファイル"第21回日本口腔腫瘍学会総会・学術大会. 97 (2003)

Junyoshi Mizoe、Shinji Yamamoto、Yunao Kozu、Morihiro Higo、Maki Kato、Naohiko Seki、Masaki Takiguchi：“使用口腔鳞状细胞癌衍生细胞系进行辐射和重离子照射的基因表达谱”第 21 届日本口腔肿瘤学会会员大会和学术会议会议。97（2003）

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

丹沢秀樹, 溝江純悦, 鵜澤一弘, 椎葉正史, 柴原孝彦, 山本信治: "悪性腫瘍と正常上皮における重粒子線によるDNA構造変化の検討"平成14年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書. 170-171 (2003)

Hideki Tanzawa、Junyoshi Mizoe、Kazuhiro Uzawa、Masashi Shiiba、Takahiko Shibahara、Shinji Yamamoto：“重离子束在恶性肿瘤和正常上皮中引起的 DNA 结构变化的检查” 2002 年国立放射线科学研究所，重离子束癌症治疗研究报告关于设备的联合使用等 170-171 (2003)