精神分裂病関連遺伝子操作動物の作製とその行動および脳内分子の解析

1)。我们正在建立利用CaMKTI启动子在前脑区过度表达MRT-1b基因的转基因小鼠系。在四个转基因品系中，有两个已经证实了生殖系基因的传递。现在我们正在培育F2代的小鼠。我们利用酵母双杂交系统克隆了6个与mrt1b相互作用的蛋白。用免疫共沉淀法证实了相互作用。该方法将HA标记的mrt1b克隆和Myc标记的克隆共转染HEK293细胞，并用各自的抗体进行免疫共沉淀。这六个克隆中有四个是未知的。在已知的基因中，一个属于磷酸酶家族，另一个具有SAM结构域和PD结构域。

1). We are establishing the transgenic mice line which express mrt-1b gene excessively in the forebrain area using CaMKTI promoter. Germline gene transmission has been confirmed in two of the four transgenic lines. Now we are breeding the F2 generation of the mice.2). We have cloned six proteins which interact with Mrt1b using the yeast two-hybrid system. The interactions were confirmed by coimmunoprecipitation method. In this method, HA-tagged Mrt1b and Myc-tagged clones were cotransfected into HEK293 mammalian cells and were coimmunoprecipitated by each antibodies. Four of the six clones were unknown. Among the known genes, one belongs to the phosphatase family and the other has a SAM domain and a PD domain.