The role of bone marrow transplantation for the ischemic hepatic injury and regeneration

骨髓移植在缺血性肝损伤及再生中的作用

• 批准号: 14571215
• 负责人: TSUCHIYA Takao
• 金额: $ 2.24万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571215/
• 关键词: hepatic regeneration; ischemic; reperfusion injury; bone marrow transplantation; green mouse; anti-GFP antibody

1. After long time ischemia/reperfusion of the liver, zonal necrosis area appeared at the central vein area. After that, necrosis areas spread2. Bone marrow cells from mice transgenic for green fluorescence protein (GFP) were transplanted to the B6 mice irradiated X-ray at a dose of 12Gy to make chimeric mice. After hepatic I/R, GFP positive cell were detected at the hepatic parenchyma and vascular endothelial cells. We suggested that hepatic endothelial cells might be replaced with these GFP positive cells at the damaged liver.

1.肝脏长时间缺血再灌注后，中央静脉区出现带状坏死区。之后，坏死区域扩散2。将转绿色荧光蛋白（GFP）基因小鼠的骨髓细胞移植到经12 Gy X射线照射的B6小鼠体内，制备嵌合体小鼠。肝I/R后，肝实质和血管内皮细胞可见GFP阳性细胞。我们认为，肝内皮细胞可能被替换为这些绿色荧光蛋白阳性细胞在受损的肝脏。

项目成果

Ishii S, Sato Y, Terashima M, Saito T, Suzuki S, Murakami S, Gotoh M.: "A novel method for determination of ATP, ADP, and AMP contents of a single pancreatic islet before transplantation."Transplant Proc (in press).

Ishii S、Sato Y、Terashima M、Saito T、Suzuki S、Murakami S、Gotoh M.：“一种在移植前测定单个胰岛 ATP、ADP 和 AMP 含量的新方法。”Transplant Proc（出版中）

Tsuchiya T, Abe T, Saito T, Sato Y, Kenjo A, Yamada F, Asari S, Nishi I, Tanaka K, Gotoh M: "The successful living donor liver transplantation in patient with preoperative fungal infection."Transplant Ploc. 35(1). 401 (2003)

Tsuchiya T、Abe T、Saito T、Sato Y、Kenjo A、Yamada F、Asari S、Nishi I、Tanaka K、Gotoh M：“术前真菌感染患者成功的活体肝移植。”移植 Ploc。

Matsuyama S, Gunji T, IseK, Satoh Y, Sitoh T, Gotoh M: "Permanent acceptance of mitomicineC-treated islet allograft."Transplantation. 76(1). 65-71 (2003)

Matsuyama S、Gunji T、IseK、Satoh Y、Sitoh T、Gotoh M：“永久接受丝裂霉素 C 处理的胰岛同种异体移植物。”移植。

後藤満一, 齋藤拓朗: "再生医学 今後の発展と臨床との接点"Surgery Frontier. 10. 229-230 (2003)

Manichi Goto、Takuro Saito：“再生医学的未来发展及其与临床实践的接口”Surgery Frontier 10. 229-230 (2003)。

Matsuyama S, Gunji T, Ise K, Sato Y, Saito T, Gotoh M.: "Permanent acceptance of mitomycin C-treated islet allograft"Transplantation. 76(1). 65-71 (2003)

Matsuyama S、Gunji T、Ise K、Sato Y、Saito T、Gotoh M.：“永久接受丝裂霉素 C 处理的胰岛同种异体移植物”移植。