Potential of primary care of multiple organ failure by gene analyses of characteristics of enterotoxin and endotoxin production in mixed infection of MTRSA and E. coli and fundamental examination

MTRSA和大肠杆菌混合感染肠毒素和内毒素产生特征的基因分析及基础检查对多器官功能衰竭初级保健的潜力

• 批准号: 14571237
• 负责人: SUMIYAMA Y
• 金额: $ 2.3万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571237/
• 关键词: MRSA; sepsis; mouse; anti TSST-1 antibody; IL-1; IFN-γ; bacterialtranslocation; TSST-1; E.coli; エンドトキシン; 内因性感染; MRSA腸炎; 多臓器不全; 大腸癌; E.coil; エンドトキシ; マウスモデル; エンテロトキシン

We made gut-derived sepsis with MRSA in mice. The bacterial suspension was orally administered between days 1 and 3. To aid the colonization of MRSA, the normal intestinal flora of the mice was disturbed by administering ampicillin (ABPC) by subcutaneous injection daily between days 1 and 3. After the administration of bacteria, mice were then given 250 mg/kg of cyclophosphamide (CY) by intraperitoneal injection on day 1, 4 and 7.We studied the efficacy of antiTSST-1 monoclonal antibody against gut-derived sepsis with MRSA.Although the significant difference was not recognized, antiTSST-1 monoclonal antibody administrated group tended to show highly survival rate.We also studied about host defense. WT mice, IL-1 KO and IFN-γ KO mice were used in gut-derived sepsis with MRSA. Although WT mice were survived more than 80%, IL-1 KO mice died all. The numbers of viable bacteria in organs were significantly high level in the IL-1 KO mice.IFN-γ KO mice were tend to die than WT mice.IL-1 and IFN-γ were indicated that they were one of important host defense cytokines.

我们用MRSA制作了小鼠的肠源性败血症。为了促进MRSA的定植，在第1~3天每天皮下注射氨苄西林(ABPC)，扰乱小鼠的正常肠道菌群。给菌后第1、4、7天，小鼠腹腔注射环磷酰胺(CY)250 mg/kg。我们研究了抗TSST-1单抗对MRSA肠源性败血症的疗效，虽然没有明显差异，但抗TSST-1单抗给药组有很高的存活率。WT小鼠、IL-1KO和干扰素-γKO小鼠用于MRSA肠源性脓毒症。虽然WT小鼠存活80%以上，但IL-1 KO小鼠全部死亡。IL-1KO小鼠脏器中的活菌数明显高于WT小鼠，IL-1和γ-γ是重要的宿主防御细胞因子之一。