Potential of primary care of multiple organ failure by gene analyses of characteristics of enterotoxin and endotoxin production in mixed infection of MTRSA and E. coli and fundamental examination

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We made gut-derived sepsis with MRSA in mice. The bacterial suspension was orally administered between days 1 and 3. To aid the colonization of MRSA, the normal intestinal flora of the mice was disturbed by administering ampicillin (ABPC) by subcutaneous injection daily between days 1 and 3. After the administration of bacteria, mice were then given 250 mg/kg of cyclophosphamide (CY) by intraperitoneal injection on day 1, 4 and 7.We studied the efficacy of antiTSST-1 monoclonal antibody against gut-derived sepsis with MRSA.Although the significant difference was not recognized, antiTSST-1 monoclonal antibody administrated group tended to show highly survival rate.We also studied about host defense. WT mice, IL-1 KO and IFN-γ KO mice were used in gut-derived sepsis with MRSA. Although WT mice were survived more than 80%, IL-1 KO mice died all. The numbers of viable bacteria in organs were significantly high level in the IL-1 KO mice.IFN-γ KO mice were tend to die than WT mice.IL-1 and IFN-γ were indicated that they were one of important host defense cytokines.