Potential of primary care of multiple organ failure by gene analyses of characteristics of enterotoxin and endotoxin production in mixed infection of MTRSA and E. coli and fundamental examination

我们用MRSA在小鼠身上制造了肠道源性败血症。细菌悬浮液在第1天和第3天口服。为了帮助MRSA的定植，在第1天至第3天每天皮下注射氨苄西林（ABPC）来干扰小鼠的正常肠道菌群。细菌给药后，小鼠于第1、4、7天腹腔注射250 mg/kg环磷酰胺（CY）。我们研究了抗tsst -1单克隆抗体对MRSA肠道源性脓毒症的疗效。抗tsst -1单克隆抗体给药组虽无明显差异，但生存率较高。我们还学习了宿主防御。WT小鼠，IL-1 KO和IFN-γ KO小鼠用于MRSA肠道源性败血症。WT小鼠存活率超过80%，IL-1 KO小鼠全部死亡。IL-1 KO小鼠脏器内活菌数量显著增高。IFN-γ KO小鼠比WT小鼠更容易死亡。IL-1和IFN-γ是重要的宿主防御因子之一。

We made gut-derived sepsis with MRSA in mice. The bacterial suspension was orally administered between days 1 and 3. To aid the colonization of MRSA, the normal intestinal flora of the mice was disturbed by administering ampicillin (ABPC) by subcutaneous injection daily between days 1 and 3. After the administration of bacteria, mice were then given 250 mg/kg of cyclophosphamide (CY) by intraperitoneal injection on day 1, 4 and 7.We studied the efficacy of antiTSST-1 monoclonal antibody against gut-derived sepsis with MRSA.Although the significant difference was not recognized, antiTSST-1 monoclonal antibody administrated group tended to show highly survival rate.We also studied about host defense. WT mice, IL-1 KO and IFN-γ KO mice were used in gut-derived sepsis with MRSA. Although WT mice were survived more than 80%, IL-1 KO mice died all. The numbers of viable bacteria in organs were significantly high level in the IL-1 KO mice.IFN-γ KO mice were tend to die than WT mice.IL-1 and IFN-γ were indicated that they were one of important host defense cytokines.