RESEARCH FOR PSEUDOCAPSULE FORMATION OF METASTATIC LIVER TUMORS

目的：为明确肝肿瘤周围假包膜的形成原因及发病机制，对结直肠癌肝转移瘤进行病理形态学及DNA芯片分析。材料与方法：从结直肠癌肝肿瘤切除术冰冻标本中，采用激光显微解剖法从假包膜和非癌肝实质附近的癌组织中收集总RNA。选择肿瘤有厚包膜的5例和肿瘤无包膜的5例分别进行分析。分析采用genesspring和d-Chip软件。结果：通过genesspring软件分析，与假包膜形成可能相关的基因在癌组织中比在正常组织中更常见。d-Chip挑选出47个基因，这些基因在癌组织和非癌组织中表现出不同的表达水平。

PURPOSE : To clarify the cause and pathogenesis of pseudocapsule around the liver tumor, liver metastatic tumor from colorectal cancer were analyzed pathomorphologically and by DNA microarray. MATERIAL AND METHODS : 1.Harvesting of tissue RNA and DNA microarray analysis From the frozen specimen of resected liver tumor from colorectal cancer, total RNA was collected by Laser microdissection from cancer tissue near the pseudocapsule and noncancerous liver parenchyma. Five patients whose tumor had thick capsule and the other five whose tumor showed no capsule were selected for analysis, respectively. For analysis, GeneSpring and d-Chip software was used. RESULTS : From the analysis using GeneSpring software the genes possibly related to pseudocapsule formation were more frequently found in the cancer tissue than in the normal tissue. The d-Chip picked out 47 genes which showed the different level of expression between cancer and noncancerous tissues.