RESEARCH FOR PSEUDOCAPSULE FORMATION OF METASTATIC LIVER TUMORS

肝转移肿瘤假包膜形成的研究

• 批准号: 14571250
• 负责人: YAMAMOTO Junji
• 金额: $ 2.24万
• 依托单位: JAPANESE FOUNDATION FOR CANCER
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571250/
• 关键词: colorectal liver metastasis; PSEUDOCAPSULE; DNA microarray; pathology; 肝腫瘍; 腫瘍-非腫瘍間線維組織; 血管侵襲; 転移性肝腫瘍; 胃癌; 大腸癌; 切除後予後

PURPOSE : To clarify the cause and pathogenesis of pseudocapsule around the liver tumor, liver metastatic tumor from colorectal cancer were analyzed pathomorphologically and by DNA microarray. MATERIAL AND METHODS : 1.Harvesting of tissue RNA and DNA microarray analysis From the frozen specimen of resected liver tumor from colorectal cancer, total RNA was collected by Laser microdissection from cancer tissue near the pseudocapsule and noncancerous liver parenchyma. Five patients whose tumor had thick capsule and the other five whose tumor showed no capsule were selected for analysis, respectively. For analysis, GeneSpring and d-Chip software was used. RESULTS : From the analysis using GeneSpring software the genes possibly related to pseudocapsule formation were more frequently found in the cancer tissue than in the normal tissue. The d-Chip picked out 47 genes which showed the different level of expression between cancer and noncancerous tissues.

目得：为探讨肝转移瘤周围假包膜的形成原因及发病机制，对大肠癌肝转移瘤进行病理形态学及基因芯片分析。材料和方法：1.组织RNA的提取及DNA微阵列分析从结直肠癌肝肿瘤冰冻标本中，采用激光显微切割技术，从癌组织中提取总RNA。分别选取5例肿瘤有厚包膜和5例肿瘤无包膜的患者进行分析。为了分析，使用GeneSpring和d-Chip软件。研究结果：从使用GeneSpring软件的分析中，可能与假包膜形成相关的基因在癌组织中比在正常组织中更频繁地被发现。D-Chip筛选出47个基因，这些基因在癌组织和非癌组织中表达水平不同。

项目成果

Successful outcome after resection of pancreatic cancer with a solitary hepatic metastasis

孤立性肝转移胰腺癌切除后取得成功

• 发表时间: 2004
• 期刊: Hepatogastroenterology 51・56
• 作者: Shimada K;Kosuge T;Yamamoto J;Yamasaki S;Sakamoto M.
• 通讯作者: Sakamoto M.

Sakamoto Y, Yamamoto J, Kokudo N, et al.: "Bloodless liver resection using the monopolar floating ball plus ligasure diathermy : preliminary results of 16 liver resections"World Journal of Surgery. 28・2. 166-172 (2004)

Sakamoto Y、Yamamoto J、Kokudo N 等人：“使用单极浮球加结扎透热疗法的无血肝脏切除术：16 例肝脏切除术的初步结果”，《世界外科杂志》28・2（2004 年）。

Surgical resection of pulmonary metastases from colorectal cancer : indicators for patient selection

结直肠癌肺转移的手术切除：患者选择指标

• 期刊: JpnJ Clin Oncol (in print)
• 作者: Koga R;Yamamoto J;Saiura A;et al.
• 通讯作者: et al.

山本順司, 齋浦明夫: "2.肝移植治療-切除療法と比較して"外科. 65・8. 914-918 (2003)

Junji Yamamoto、Akio Saiura：“2. 肝移植治疗 - 与切除治疗的比较”Surg. 914-918 (2003)。

山本順司, 齋浦明夫, 阪本良弘, 他: "胃癌肝転移"早期大腸癌. (5月号). (2004)

Junji Yamamoto、Akio Saiura、Yoshihiro Sakamoto 等：“胃癌肝转移”早期结直肠癌（2004 年 5 月号）。