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spinal reconstruction using recombinant human bone morphogenic protein after total spondylectomy

全脊椎切除术后使用重组人骨形态发生蛋白进行脊柱重建

基本信息

批准号：
14571366
负责人：
KAWAHARA Norio
金额：
$ 2.24万
依托单位：
KANAZAWA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571366/
关键词：
bone morphogenetic protein spinal tumor spinal fusion 脊椎固定

项目摘要

20 Dogs underwent total spondylectomy of TI3 and spinal reconstruction with titanium mesh cage using autogeneous bone graft(resected vertebral body) and recombinant human bone morphogenetic protein-2(rhBMP-2) to evaluate bone remodeling and fusion.Control group(autogeneous bone graft n=10):had the titanium cage reconstruction filled with autogenous bone, and BMP-treated group(n=10):had reconstruction with the titanium cage filled collagen sponge soaked with rhBMP-2. 5 dogs of each group were killed 8weeks(group A) and 16 weeks(group B) after the surgery and we evaluated bone remodeling and fusion by imaging test(Xp, CT scan) and histologic examination.Imaging test revealed bone fusion in all specimens with the exception of one pseudoarthrosis in the control group. Histologic examination showed 4 of 5 dogs in control group A had normal lamella of trabecular bone formation from the endplate of the adjacent vertebral bodies towards the center of the cage, and woven bone was present around the center of cage. It is considered to be a progress of remodeling. The other one dog had pseudoarthrosis. 3 of 5 dogs in control group B achieved consecutive trabecular cancellous bony fusion between adjacent vertebral bodies. The other two had more progressive abone formation towards the center of the mesh compared to the control group A. It is considered that bone fusion is going to be progressed.In BMP-treated group A, there were progressive bone formation towards the center of the mesh. All the 5 dogs in BMP-treated group B achieved consecutive trabecular cancellous bony fusion between the grafted bone and adjacent vertebral bodies.Both groups could have favorable bone fusion, but BMP-treated group had more trabecular bone formation throughout the length of the mesh than control group.This study confirms rhBMP2 augment bony fusion and remodeling on vertebral reconstruction after total spondylectomy.

20只犬行TI 3全脊椎切除，钛网融合器重建，自体植骨（切除椎体）和重组人骨形态发生蛋白-2（rhBMP-2）联合应用，评价骨重建和融合情况，对照组（自体植骨组，n=10）：钛网融合器内填充自体骨，BMP治疗组（n=10）：钛网融合器内填充rhBMP-2浸泡的胶原海绵。术后8周（A组）和16周（B组）每组各取5只犬，通过X线、CT扫描和组织学检查评价骨重建和融合情况。组织学检查显示，对照组A的5只犬中有4只从相邻椎体终板向融合器中心具有正常的骨小梁形成，融合器中心周围存在编织骨。它被认为是一个重塑的过程。另一只犬有假关节。对照组B 5只犬中有3只在相邻椎体之间实现连续的小梁松质骨融合。与对照组A相比，另外两个组的骨形成朝向补片中心更渐进。BMP处理组A中，有向网状物中心的进行性骨形成。BMP治疗组B中的所有5只狗均在移植骨与邻近椎体之间实现了连续的松质骨融合。两组均能实现良好的骨融合，但BMP治疗组在整个网状物长度上的松质骨形成多于对照组。该研究证实了rhBMP 2增强了全脊椎切除术后椎骨重建的骨融合和重建。