Basic Study for the development of artificial hip joint -Development of new bone-prosthesis interface -

在负重条件下对一种名为“多层网状”的新型髋关节假体多孔涂层进行了测试。将羟基磷灰石(HA)涂层多层网状物和常规微珠植入犬右侧髋关节，分别于植入后3、6、10周、6、12个月处死动物。通过顶出试验评价种植体与骨之间的剪切强度。根据横切面的背向散射电子成像扫描电子显微镜(BEI-SEM)图像计算骨长入。甲苯胺蓝染色切片和BEI-SEM图像进行组织学评价。每次测试时，HA涂层多层网状种植体的平均推出强度均大于珠状涂层的，且HA涂层多层网状种植体在3周和6周时的推出强度显著高于珠状涂层的种植体(p&lt；0.05)。HA涂层的多层网状种植体的强度在6个月和12个月时甚至更高，而强度…更多的包裹珠子的样品减少了。除6个月外，HA涂层多层网状种植体的骨内长出百分率均显著高于珠状涂层种植体(p&lt；0.05)。在6个月和12个月时，HA涂层多层网状种植体的骨生长数据增加，而珠状涂层植入体的骨生长数据减少。3周时，新生骨形成已达到羟基磷灰石涂层多层网状物表面多孔区的底部，但尚未达到常规微珠表面的底部。6个月和12个月时，骨小梁表面较小的孔洞阻止了骨小梁的增厚，12个月时，断裂部位在骨珠表面的骨-种植体界面，而在HA涂层的多层网状物表面的骨侧。在12个月时，骨折部位之间的差异有统计学意义(p&lt；0.05)。羟基磷灰石涂层的多层网状支架比传统的珠状支架提供了更快、更坚固和更耐用的骨整合。较少

A new type of porous coating for hip prostheses called "multilayered mesh" was tested under weight bearing conditions. The hip stems of hydroxyapatite (HA)-coated multilayered mesh and conventional beads were implanted into canine right hips, and animals were killed 3, 6 and 10 weeks and 6 and 12 months after implantation. Shear strength between the implant and the bone was evaluated by the push-out test. Bone ingrowth was calculated from backscattered electron imaging-scanning electron microscopy (BEI-SEM) images of transverse sections. Toluidine blue stained sections and the BEI-SEM images were evaluated histologically. The mean push-out strength of the HA-coated multilayered mesh samples was greater than that of the beads coated samples every time tested, and the HA-coated multilayered mesh implants had significantly stronger push-out strength at 3 and 6 weeks (p<0.05). The strength of the HA-coated multilayered mesh implants was even greater at 6 and 12 months, whereas the strength … More of the beads-coated samples decreased. The HA-coated multilayered mesh implants showed significantly higher percentages of bone ingrowth than the beads coated implants every time tested, except at 6 months (p<0.05). At 6 and 12 months, the bone ingrowth data for the HA-coated multilayered mesh implants increased, whereas it decreased for the beads coated implants. The new bone formation had reached the bottom of the porous area of the HA-coated multilayered mesh surface by 3 weeks, but not had reached the bottom of the conventional beads surface. At 6 and 12 months, the smaller pores of the bead surface stopped the thickening of trabecular bane, and at 12 months, the break sites were at the bone-implant interface of the bead surface, whereas they were on the bone side of the HA-coated multilayered mesh surface. The difference between the break sites was significant at 12 months (p<0.05). The HA-coated multilayered mesh stem provided faster, stronger, and more durable osseointegration than the conventional bead stem. Less