Effect of nicorandil on cardiac function in sepsis

尼可地尔对脓毒症心功能的影响

• 批准号: 14571465
• 负责人: SERITA Ryohei
• 金额: $ 2.24万
• 依托单位: KEIO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571465/
• 关键词: nicorandil; Sepsis; Cardiac Function; K-ATP channel; Working heart; サイトカイン; アポトーシス

The purposes of present study were to determine the effects of nicorandil on cardiac mechanical function and cardiac efficiency as well as mortality rate and cytokine production in septic rats.Cardiac function : The cardiac output, cardiac work, and cardiac efficiency in the both cecal ligation and perforation (CLP) groups significantly decreased as compared with the both Sham groups. In the Sham group, no significant differences of myocardial function were observed between the subgroups. The cardiac output, cardiac work, and cardiac efficiency in nicorandil treated CLP group were kept higher than controlled CLP group.Survival Rate : Survival rate of nicorandil treated CLP group significantly improved as compared with control group. Median times of mortality were 29 (95% CI: 26.9-31.1) hours versus 24 (95% CI: 23.1-24.9) hours, respectively.Biochemical analyses : Plasma concentration of TNF-α significantly decreased in nicorandil treated CLP group as compared with controlled CLP group at 21 hours after operation, whereas there were no significant differences between the groups in that of IL 1-β and MIF. Plasma concentration of nitrate/nitrite significantly decreased in nicorandil treated CLP group as compared with controlled CLP group at 21 hours after operation.Apoptosis : The apoptotic cells were observed about 1% of cardiomyocyte in the both groups. There was no significant difference between the groups.Conclusion : The present study indicates that the treatment with nicorandil improve cardiac function and survival rate in experimental sepsis model.

本研究的目的是确定尼可地尔对脓毒症大鼠心脏机械功能和心脏效率以及死亡率和细胞因子产生的影响。心脏功能：盲肠结扎穿孔（CLP）组的心输出量、心脏作功和心脏效率与两个Sham组相比显著降低。Sham组各亚组间心肌功能无明显差异。尼可地尔治疗CLP组的心输出量、心功、心效率均高于对照组。生存率：尼可地尔治疗CLP组的生存率较对照组显著提高。中位死亡时间为29（95% CI：26.9-31.1）小时vs 24小时（95% CI：23.1-24.9）小时。术后21 h尼可地尔治疗CLP组血浆TNF-α浓度较对照CLP组显著降低，而IL 1-β和MIF在各组间无显著性差异。术后21 h尼可地尔治疗组血浆硝酸盐/亚硝酸盐浓度较对照组显著降低（P <0.05）。细胞凋亡：两组心肌细胞均可见约1%的凋亡细胞。结论：尼可地尔治疗脓毒症可改善心功能，提高生存率。