The study of cell membrane ion channels involving in automaticity of human uterine smooth muscle cells.

细胞膜离子通道参与人子宫平滑肌细胞自律性的研究。

• 批准号: 14571596
• 负责人: INOUE Yoshihito
• 金额: $ 1.34万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571596/
• 关键词: uterine smooth muscle.; hyperpolarization-activated inward current; T-type calcium channel; voltage clamp; α1 subunit; ヒト子宮平滑筋; T-type Ca^<2+> channel; 収縮頻度調整; α1 subunit; 過分極誘発性内向き電流; 単離細胞

The aim of this study was to find the membrane channels which involves in automaticity of human myometrium. For the beginning, we tried to record hyperpolarization-induced inward current which was recorded in rat myometrium using patch clamp methods, but it failed. So we evaluated the difference in the expression of mRNA of two types of calcium channels between longitudinal and circular muscle layer of rat mayometrium during pregnancy. Changes in the expressions of the mRNA encoding L-type (α1C) and T-type (α1G, α1H, and all) calcium channels in longitudinal and circular smooth muscle cells of the rat myometrium were examined using a comparative kinetic RT/PCR method. During the course of pregnancy, α1C mRNA expression showed an N-shaped change in longitudinal muscle, but simply increased after mid pregnancy in circular muscles. The mRNAs for α1G and α1H, but not that for all, were expressed in both longitudinal and circular smooth muscle. In longitudinal muscle, the changes in α1H mRNA was similar to that in α1C mRNA during gestation, but the expression of α1G mRNA changed significantly only at term (day 22). In circular muscle, α1H mRNA expression was stable at any stage during pregnancy, but α1G mRNA significantly increased on day 15 and at term. No relationship was observed between voltage-dependent calcium-channel mRNA expressions and either proliferation or hypertrophy of circular muscle during pregnancy. These results show, (a)that during pregnancy, the expression levels of L-type channels change dynamically, and it may contribute directly to the regulation of cell excitability, and (b)that the T-subtype that increases during pregnancy differs between longitudinal and circular muscle cells, although their functions remain unclear.

这项研究的目的是找到涉及自动肌层自动化的膜通道。一开始，我们尝试记录超极化诱导的内向电流，该电流使用斑块夹方法记录在大鼠肌层中，但失败了。因此，我们评估了妊娠期间大鼠Mayomortim的纵向和圆形肌肉层之间两种类型的钙通道的mRNA表达差异。使用比较的动力学RT/PCR方法检查了大鼠肌层纵向和圆形平滑肌细胞中大鼠肌层平滑肌细胞中编码L型（α1C）和T型（α1G，α1H和所有）钙通道的表达的变化。在怀孕过程中，α1cmRNA表达显示纵向肌肉的N形变化，但在圆形肌肉中怀孕后仅增加。 α1G和α1H的mRNA，但不是所有的mRNA在纵向和圆形平滑肌中表达。在纵向肌肉中，α1HmRNA的变化与妊娠期间α1cmRNA中的变化相似，但是α1gmRNA的表达仅在学期（第22天）发生明显变化。在圆形肌肉中，α1HmRNA表达在妊娠期间的任何阶段都稳定，但是α1gmRNA在第15天和期间显着增加。在怀孕期间，依赖电压依赖性的钙通道mRNA表达与圆形肌肉的增殖或肥大之间未观察到任何关系。这些结果表明，（a）在怀孕期间，L型通道的表达水平动态变化，并且可能直接有助于细胞兴奋性的调节，并且（b）纵向和圆形肌肉细胞之间增加的T-屈服量差异，尽管它们的功能仍然不清楚。

项目成果

Amino acid-permeable anion channels in early mouse embryos and their possible effects on clevage.

早期小鼠胚胎中氨基酸可渗透的阴离子通道及其对卵裂的可能影响。

• 发表时间: 2003
• 期刊: Biology of Reproduction 68
• 作者: Sonoda M;Okamoto F;Kajiya H;Inoue Y Honjo K;Sumii Y;Kawarabayasi T;Okabe K
• 通讯作者: Okabe K

Diversity of inhibitory responses to β2-stimulants shown by term-pregnant human myometria in vitro is partly due to differences in receptor density

足月妊娠人类子宫肌层在体外显示的对 β2 兴奋剂的抑制反应的多样性部分是由于受体密度的差异

• 发表时间: 2002
• 期刊: American Journal of Obstetrics and Gynecology 186
• 作者: Sakakibara T;Inoue Y;Uzue S et al.
• 通讯作者: Uzue S et al.

Amino acid-permeable anion channels in early mouse embryos and their possible effects on cleavage

早期小鼠胚胎中氨基酸可渗透的阴离子通道及其对卵裂的可能影响

• 发表时间: 2003
• 期刊: Biology of Reproduction 68
• 作者: Sonoda M;Okamoto F;Kajiya H;Inoue Y et al.
• 通讯作者: Inoue Y et al.

Differential expression of L- and T-type calcium channels between longitudinal and circular muscles of the rat myometirium during pregnancy

妊娠期大鼠子宫肌层纵肌和环肌L型和T型钙通道差异表达

• 发表时间: 2005
• 期刊: Gynecologic and Obstetric Investigation 59
• 作者: Ohkubo T;Inoue Y;Kawarabayashi T;Kitamura K
• 通讯作者: Kitamura K

Diversity of inhibitory responses to β2-stiinulants shown by term-pregnant human myometria in vitro is partly due to differences in receptor density.

足月妊娠人类子宫肌层在体外表现出的对β2-兴奋剂的抑制反应的多样性部分是由于受体密度的差异。

• 发表时间: 2002
• 期刊: Am J Obstet Gynecol 186
• 作者: Sakakibara T;Inoue Y;Uzue S;Tsukamoto T;Kobayashi M;Kojima M;Akahane M;Kitamura K;Kawarabayashi T
• 通讯作者: Kawarabayashi T