The induction of cytotoxic(killer) T lymphocytes against the tumor of neck by new culture method.

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We studied the specificity of cytotoxic T lymphocytes(CTLs) which were induced in mixed lymphocyte cultures(MLC) in vitro. We used the unique culture system that bone marrow(BM) cells from responder mice as accessory cells were added to the cultures, which contained both responder lymph node cells and allogeneic stimulator spleen cells. In this report, two different stimulatory dendritic cell(DC) populations were prepared from spleen cells, such as DEC-205+ cell depleted(Mac-1+ cell enriched;MDC) or Mac-1+ cell depleted(DEC-205+ cell enriched;LDC) spleen cells.In allo-MLC, the CTL induced by the stimulation of MDC had the dual specificity for both allo major histocompatibility antigen complex(MHC) and allo X-chromosome linked gene products(XLGP) of stimulator cells. On the other hand, the CTL induced by the stimulation of LDC had the specificity for allo MHC alone.In polyclonal CTL induction by concanavalin A, autologous, not allogeneic, spleen cells were used as stimulator cells. The CTL induced by MDC-stimuli had the dual specificity, such as polyclonal allo-MHC and stimulator-self-XLGP. On the contrary, when LDC-stimuli was used, the CTL having the dual specificity were specifically suppressed.We already reported that the CTL induced by the stimulation with P815 tumor cells(originated from DBA/2 mice), using these culture systems, had the dual specificity, that is, H-2d(MHC of DBA/2 mice) and P815 tumor specific antigens(TSA). P815 TSA behaved like the XLGP, but were different one.However, this TSA had the same antigenic function with XLGP. The conclusion in this report suggested the possibility that some(MDC type) tumor cells could induce the CTL having dual specificity, MHC and XLGP-like-TSA, the other hand, other(LDC type) tumor cells could suppress or tolerate the induction of CTL having this dual specificity.