The development for control of hypersensitivity with nasal allergy for usinge explant culture technique

外植体培养技术控制鼻过敏超敏反应的进展

• 批准号: 14571642
• 负责人: OKUBO Kimihiro
• 金额: $ 1.54万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571642/
• 关键词: neuropeptide; substance P; allergic rhinitis; airway hyper-reactivity; allergen challenge; pollinosis; 気道過敏性亢進; 好酸球; 鼻アレルギー; ニュートラルエンドペプチデース; 神経ペプチド分解酵素

Allergic reaction of the nose comprises of an immediate and late reaction same as bronchi. In the first study, we performed the analysis of eosinophil migration using ex vivo culture system in response to allergen to elucidate the late phase response in allergic rhinitis. The late phase of allergen response in ex vivo culture, eosinophil migration was up-regulated by inhibition of neuropeptide peptidases. Moreover, extrinsic substance P(SP) may up-regulates the eosinophil migration into culture medium at late phase. In the second study, the analyses of the nasal symptoms by recurrent allergen exposure for perennial allergic rhinitis were performed. The nasal reactivity of each symptom was increased by recurrent allergen exposure. In the third experiment, the manifestation of neuro-related substances in the nasal surface by allergen exposure was analyzed. NK-1 receptor, neutral endopeptidase, and endothelin mRNA was increased after allergen challenge in case of positive challenge reaction. The results from these studies indicate that nasal hyper-reactivity was existed after allergen exposure, and SP has some role on this hyper-reactivity including the action of eosinophil migration in allergic nasal mucosa, and also indicates protease inhibitors control this hyper-reactivity via inhibition of SP.

与支气管一样，鼻的过敏反应也包括即时反应和迟发反应。在第一项研究中，我们使用体外培养系统对嗜酸性粒细胞迁移进行了分析，以阐明过敏性鼻炎的晚期反应。在离体培养中过敏原反应的晚期，嗜酸性粒细胞迁移通过抑制神经肽肽酶而上调。此外，外源性P物质（SP）可在晚期上调嗜酸性粒细胞向培养液中的迁移。在第二项研究中，对常年性变应性鼻炎的鼻症状进行了反复过敏原暴露分析。每种症状的鼻反应性随着过敏原的反复暴露而增加。在第三个实验中，分析了过敏原暴露后鼻表面神经相关物质的表现。在激发反应阳性的情况下，过敏原激发后NK-1受体、中性内肽酶和内皮素mRNA增加。这些研究结果表明，过敏原暴露后存在鼻高反应性，并且SP在这种高反应性中具有一定的作用，包括过敏性鼻粘膜中嗜酸性粒细胞迁移的作用，并且还表明蛋白酶抑制剂通过抑制SP来控制这种高反应性。

项目成果

Kimihiro Okuda: "6th International Academic Conference on Immuno-and Molecular Biology in Otolaryngology"Kugler Publications(未定). (2003)

Kimihiro Okuda：“第六届耳鼻喉科免疫和分子生物学国际学术会议”Kugler Publications（待定）（2003 年）。

Repeated antigen challenge in patients with perennial allergic rhinitis to house dust mite.

常年性过敏性鼻炎患者对屋尘螨进行重复抗原激发。

• 发表时间: 2003
• 期刊: Allergology International 52
• 作者: Gotoh M;Okubo K
• 通讯作者: Okubo K

The effect of substance P on the eosinophil migration of allergic nasal mucosa.

P物质对过敏性鼻粘膜嗜酸性粒细胞迁移的影响。

• 期刊: Allergology International (In press)
• 作者: Okubo K;Gotoh M;Yagi T
• 通讯作者: Yagi T