The development for control of hypersensitivity with nasal allergy for usinge explant culture technique

鼻子的过敏反应与支气管一样，包括立即反应和晚发反应。在第一项研究中，我们利用体外培养系统分析了嗜酸性粒细胞对过敏原的迁移，以阐明变应性鼻炎的晚期反应。在离体培养的变应原反应后期，嗜酸性粒细胞的迁移通过抑制神经肽肽酶而上调。此外，外源P物质（SP）可能在后期上调嗜酸性粒细胞向培养基的迁移。在第二项研究中，分析了常年性变应性鼻炎患者反复接触过敏原后的鼻症状。每一症状的鼻反应性均因反复接触过敏原而增加。第三个实验分析变应原暴露后鼻表神经相关物质的表现。阳性激射反应时，NK-1受体、中性内肽酶和内皮素mRNA均升高。这些研究结果表明，过敏原暴露后鼻腔存在高反应性，而SP对这种高反应性有一定作用，包括对过敏性鼻黏膜嗜酸性粒细胞迁移的作用，也表明蛋白酶抑制剂通过抑制SP来控制这种高反应性。

Allergic reaction of the nose comprises of an immediate and late reaction same as bronchi. In the first study, we performed the analysis of eosinophil migration using ex vivo culture system in response to allergen to elucidate the late phase response in allergic rhinitis. The late phase of allergen response in ex vivo culture, eosinophil migration was up-regulated by inhibition of neuropeptide peptidases. Moreover, extrinsic substance P(SP) may up-regulates the eosinophil migration into culture medium at late phase. In the second study, the analyses of the nasal symptoms by recurrent allergen exposure for perennial allergic rhinitis were performed. The nasal reactivity of each symptom was increased by recurrent allergen exposure. In the third experiment, the manifestation of neuro-related substances in the nasal surface by allergen exposure was analyzed. NK-1 receptor, neutral endopeptidase, and endothelin mRNA was increased after allergen challenge in case of positive challenge reaction. The results from these studies indicate that nasal hyper-reactivity was existed after allergen exposure, and SP has some role on this hyper-reactivity including the action of eosinophil migration in allergic nasal mucosa, and also indicates protease inhibitors control this hyper-reactivity via inhibition of SP.