The development for control of hypersensitivity with nasal allergy for usinge explant culture technique

鼻子的过敏反应包括与支气管炎一样的即刻和晚期反应。在第一项研究中，我们利用体外培养系统对变应性鼻炎进行了嗜酸性粒细胞迁移的分析，以阐明变应性鼻炎的晚期反应。在体外培养的过敏原反应的晚期，神经肽酶的抑制上调了嗜酸性粒细胞的迁移。此外，外源性P物质(SP)可能在培养后期上调嗜酸性粒细胞向培养基内的迁移。在第二项研究中，分析了常年性变应性鼻炎患者因反复接触变应原而出现的鼻部症状。每种症状的鼻部反应都因反复接触变应原而增加。在第三个实验中，分析了过敏原暴露对鼻表神经相关物质的影响。变应原激发阳性时，NK-1受体、中性内肽酶和内皮素m RNA表达增加。这些研究结果表明，过敏原暴露后存在鼻部高反应性，SP对这种高反应性有一定的作用，包括嗜酸性粒细胞在变态反应性鼻黏膜中的迁移，也表明蛋白酶抑制剂通过抑制SP来控制这种高反应性。

Allergic reaction of the nose comprises of an immediate and late reaction same as bronchi. In the first study, we performed the analysis of eosinophil migration using ex vivo culture system in response to allergen to elucidate the late phase response in allergic rhinitis. The late phase of allergen response in ex vivo culture, eosinophil migration was up-regulated by inhibition of neuropeptide peptidases. Moreover, extrinsic substance P(SP) may up-regulates the eosinophil migration into culture medium at late phase. In the second study, the analyses of the nasal symptoms by recurrent allergen exposure for perennial allergic rhinitis were performed. The nasal reactivity of each symptom was increased by recurrent allergen exposure. In the third experiment, the manifestation of neuro-related substances in the nasal surface by allergen exposure was analyzed. NK-1 receptor, neutral endopeptidase, and endothelin mRNA was increased after allergen challenge in case of positive challenge reaction. The results from these studies indicate that nasal hyper-reactivity was existed after allergen exposure, and SP has some role on this hyper-reactivity including the action of eosinophil migration in allergic nasal mucosa, and also indicates protease inhibitors control this hyper-reactivity via inhibition of SP.