Molecular Genetic Approach for the Virulence Factors in Prevotella

普雷沃菌毒力因子的分子遗传学方法

• 批准号: 14571756
• 负责人: OKAMOTO Masaaki
• 金额: $ 1.6万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571756/
• 关键词: Prevotella; virulence factor; genetic; periodontitis; hemolysin; 歯周病関連細菌; Prevotella属; 歯周病原性; 酵素; ホスファターゼ; 血球凝集因子; チロシンホスファターゼ

Prevotella is the most frequently isolated among the periodontopathic bacteria, however, it's virulent factors are less known. The aim of present project was to analysis the gene sequence, and its regulation of the hemagglutinin, hemolysin, hemin binding protein and phosphatases as the virulence factors in Prevotella.The hemagglutinin study in P.intermedia was done to try to express of the phg gene in Escherichia coli using the GST fusion protein system. However, the gene product did not show the hemagglutinin activity, because of forming the inclusion body. Similar sequence of the phg gene was not found among Prevotella species.Beem et al. reported that the hemagglutinin gene was cloned from the P.intermedia strain 27. We reported that this gene was found in some limited strains of P.intermediaTIGR(The Institute for Genomic Research) had reported the hemolysin gene similar to the P.melaninogenica hemolysin gene in the P.intermedia strain 17 whole genomic sequencing project. I have cloned this gene, and have expressed the hemolytic activity. Similar sequenced gene was not found in P.nigrescens. I continue to investigate the relation between the nucleotide polymorphisms of gene and the homolytic activity among P.intermedia isolated strains.We reported that the catechins from green tea inhibited several virulence factors, such as the phosphatase activities in Prevotella, and the gingipain activities in Porphyromonas. We also reported the epidemiological research of the different prevalence of the P.intermedia and P.nigrescens, which collaborated with the Beijing Capital University.

普雷沃菌是牙周病病原菌中最常见的一种，但其致病因子尚不清楚。本研究旨在分析普雷沃氏菌的血凝素、溶血素、血凝素结合蛋白和磷酸酶等毒力因子的基因序列及其调控，并以中间普雷沃氏菌的血凝素为研究对象，尝试利用GST融合蛋白系统在大肠杆菌中表达phg基因。然而，基因产物由于形成包涵体，没有表现出血凝素活性。在中间普氏菌中没有发现与phg基因相似的序列，Beem等报道从中间普氏菌27株中克隆了血凝素基因。我们报道了在一些有限的中间变形杆菌菌株中发现了该基因。TIGR（The Institute for Genomic Research）在中间变形杆菌17株全基因组测序计划中报道了与产黑色素变形杆菌溶血素基因相似的溶血素基因。我克隆了该基因，并表达了溶血活性。在黑曲霉中未发现相似的基因序列。本研究继续探讨中间青霉分离株中基因多态性与同裂酶活性的关系，发现绿色茶中的儿茶素类物质对普雷沃氏菌的磷酸酶活性和卟啉单胞菌的牙龈蛋白酶活性有抑制作用。本文还报道了与北京首都大学合作进行的中间型和变黑型疟原虫不同流行率的流行病学研究。

项目成果

Quantification of Porphyromonas gingivalis by real time PCR : new primers targeting the rgpA and rgpB gene encoding RGP

通过实时 PCR 定量牙龈卟啉单胞菌：针对编码 RGP 的 rgpA 和 rgpB 基因的新引物

• 发表时间: 2003
• 期刊: Higashi Nippon Dent.J. 22(1)
• 作者: A.Kamaguchi;R.Nakamura;M.Okamoto;et al.
• 通讯作者: et al.

Effect of quorum sensing on production of RGP (arginine specific cysteine proteinase) of Porphyromonas gingivalis

群体感应对牙龈卟啉单胞菌 RGP（精氨酸特异性半胱氨酸蛋白酶）产生的影响

• 发表时间: 2003
• 期刊: Higashi Nippon Dent.J. 22(2)
• 作者: A.Kamaguchi;R.Nakamura;T.Ohyama et al.
• 通讯作者: T.Ohyama et al.

Detection of main putative periodontopathic bacteria in subgungival plaque by polymerase chain reaction

聚合酶链反应检测龈下菌斑中主要牙周病细菌

• 发表时间: 2003
• 期刊: Chinese J.Microecol. 15(2)
• 作者: Z.Wang;M.Okamoto;S.Yang;et al.
• 通讯作者: et al.

M.Okamoto, K.-P.Leung, T.Ansai, A.Sugimoto, N.Maeda: "Inhibitory effect of green tea catechins on protein tryosine phosphatase in Prevotelle intermedia"Oral Microbiol.Immunol.. 18・3. 192-196 (2003)

M.Okamoto、K.-P.Leung、T.Ansai、A.Sugimoto、N.Maeda：“绿茶儿茶素对中间普雷沃菌中蛋白酪氨酸磷酸酶的抑制作用”口腔微生物.免疫学.. 18・3。 196（2003）

A.Kamaguchi, R.Nakamura, M.Okamoto, T.Watanabe, T.Ohyama, H.Baba: "Quantification of Poryphyromonas gigivalis by real time PCR : new primers targeting the rgpA and rgpB gene encoding RGP"Higashi Nippon Dent.J.. 22・1. 11-29 (2003)

A.Kamaguchi、R​​.Nakamura、M.Okamoto、T.Watanabe、T.Ohyama、H.Baba：“通过实时 PCR 定量吉吉瓦单胞菌：针对编码 RGP 的 rgpA 和 rgpB 基因的新引物”Higashi Nippon Dent.J .. 22・1. 11-29 (2003)