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Mechano-electrical conversion system in Merkel cell of palatine ruge

腭鲁格默克尔细胞中的机电转换系统

基本信息

批准号：
14571778
负责人：
TAZAKI Masakazu
金额：
$ 1.41万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571778/
关键词：
Merkel cell palatine ruge oral mucosa mechanoreceptor guinacrine fluorescent cell voltage-dependent calcium channel slow adaptation キナクリン蛍光細胞 CK20陽性細胞電子顕微鏡

项目摘要

To verify the mechano-electrical conversion system in Merkel cell and/or Merkel cell-neurite complexes of oral mucosa (palatine ruge), we investigated the changes in intracellular calcium concentration of single Merkel cells using microfluorimetric techniques. We also used a light, electron and confocal laser scanning microscope in order to observe the connection of MerKel cell and the nerve terminals.Results and conclusion : 1. The responses from Merkel cells-neurite complexes were slowly adapting type. Merkel cells-neurite complexes were slowly adapting type I mechanoreceptors. 2. Voltage dependent L, N, P/Q-type calcium channels and stretch activated ion channels existed in Merkel cell membrane. There is the possibility that voltage dependent L-type calcium channels can be stretch activated. Based on these results, it is suggested that Merkel cells probably function as mechano-electric transducer. However, more research of the kind of the transmitter in the Merkel cell should be necessary. 3. The Merkel cells (CK20 positive cells) were found in the bases of the epithelial thickenings in the palatine ruge. Many Merkel cells were distributed at the top of palatine ruge. Merkel cell exists in the part which is easy to receive the indentation of pressure. 4. The number of Merkel cell in the 4th palatine ruge was significantly more numerous than the 1st, 2nd and 3rd palatine ruga. 5. Indentation of continuous pressure by a denture base on palatine ruga was applied. The Merkel cells decreased in the number. 6. In the regenerative epithelium, Merkel cells were not detected, although nerve fibers (PGP 9. 5 and NSE positive, fibers) were found in experimental region throughout 7 days. The quality of a sense has the possibility to be different in the regenerative epithelium.

为了验证口腔粘膜Merkel细胞和/或Merkel细胞-轴突复合体的机电转换系统，我们用显微荧光技术研究了单个Merkel细胞内钙离子浓度的变化。并用光学显微镜、电子显微镜和激光共聚焦扫描显微镜观察Merkel细胞与神经末梢的联系。结果与结论：1.Merkel细胞-轴突复合体的反应为缓慢适应型。Merkel细胞-轴突复合体正在慢慢适应I型机械感受器。2.MERKEL细胞膜存在电压依赖性的L、N、P/Q型钙通道和拉伸激活的离子通道。电压依赖性L型钙通道有可能被牵张激活。根据这些结果，推测Merkel细胞可能具有机电转换功能。然而，应该有必要对默克尔小组中的发射器进行更多的研究。3.在腭裂上皮增厚的基底部可见Merkel细胞(CK20阳性细胞)。腭沟顶部有大量的Merkel细胞分布。默克尔槽存在于易受压痕的部位。4.第4腭沟的Merkel细胞数明显多于第1、2、3腭沟。5.使用义齿基托对腭部进行持续加压。默克尔细胞数量减少。6.在再生上皮中，未检测到Merkel细胞，但在实验区7天内可见神经纤维(Pgp 9.5和NSE阳性纤维)。在再生上皮中，感觉的性质可能会有所不同。