Mechano-electrical conversion system in Merkel cell of palatine ruge

为了验证梅克尔细胞和/或梅克尔细胞-神经突复合体的机电转换系统，我们使用微荧光技术研究了单个梅克尔细胞胞内钙浓度的变化。我们还使用光学、电子和共聚焦激光扫描显微镜观察默克尔细胞与神经末梢的连接。结果与结论：1。默克尔细胞-神经突复合物的反应是缓慢的适应类型。默克尔细胞-神经突复合物正在缓慢地适应I型机械感受器。2. 默克尔细胞膜中存在电压依赖性的L、N、P/ q型钙离子通道和拉伸激活离子通道。存在电压依赖性l型钙通道被拉伸激活的可能性。基于这些结果，我们认为默克尔细胞可能具有机电换能器的功能。然而，对默克尔细胞中的传递器进行更多的研究是必要的。3. 梅克尔细胞（CK20阳性细胞）位于腭襞上皮增厚的基底。许多默克尔细胞分布在腭骨的顶部。默克尔细胞存在于容易接受压痕的部位。4. 第4腭区默克尔细胞数量明显多于第1、2和3腭区。5. 用义齿基托在腭颊上连续压入。默克尔细胞数量减少。6. 在再生上皮中，未检测到默克尔细胞，尽管神经纤维(PGP 9。7 d内在实验区均发现NSE阳性（5和NSE阳性纤维）。感觉的质量在再生上皮中有可能是不同的。

To verify the mechano-electrical conversion system in Merkel cell and/or Merkel cell-neurite complexes of oral mucosa (palatine ruge), we investigated the changes in intracellular calcium concentration of single Merkel cells using microfluorimetric techniques. We also used a light, electron and confocal laser scanning microscope in order to observe the connection of MerKel cell and the nerve terminals.Results and conclusion : 1. The responses from Merkel cells-neurite complexes were slowly adapting type. Merkel cells-neurite complexes were slowly adapting type I mechanoreceptors. 2. Voltage dependent L, N, P/Q-type calcium channels and stretch activated ion channels existed in Merkel cell membrane. There is the possibility that voltage dependent L-type calcium channels can be stretch activated. Based on these results, it is suggested that Merkel cells probably function as mechano-electric transducer. However, more research of the kind of the transmitter in the Merkel cell should be necessary. 3. The Merkel cells (CK20 positive cells) were found in the bases of the epithelial thickenings in the palatine ruge. Many Merkel cells were distributed at the top of palatine ruge. Merkel cell exists in the part which is easy to receive the indentation of pressure. 4. The number of Merkel cell in the 4th palatine ruge was significantly more numerous than the 1st, 2nd and 3rd palatine ruga. 5. Indentation of continuous pressure by a denture base on palatine ruga was applied. The Merkel cells decreased in the number. 6. In the regenerative epithelium, Merkel cells were not detected, although nerve fibers (PGP 9. 5 and NSE positive, fibers) were found in experimental region throughout 7 days. The quality of a sense has the possibility to be different in the regenerative epithelium.