Research of the bone formation promotion effect with used together PRP from self-blood and CCEF.

自体血PRP与CCEF联合使用促进骨形成作用的研究。

• 批准号: 14571855
• 负责人: OCHI Morio
• 金额: $ 1.34万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571855/
• 关键词: Platelet-rich plasma; Osteogenesis-promot ing effect; 骨形成促進; 容量結合型電場刺激法

The aim of this study was to carry out a comparative assessment of the osteogenesis-promoting effects of platelet-rich plasma (PRP) in combination with different kinds of carrier material. We created an enclosed space by fixing a cap onto the rabbit parietal bone, in order to study osteogenesis using the guided bone regeneration (GBR) method. The space was filled with PRP along with a bone-compensating carrier to induce osteogenesis, and the amount of osteoneogenesis within the cap was measured.First, we assessed the various methods for collecting PRP. We then attached a pair of polypropylene caps to the surface of the parietal bone of each rabbit. One cap was filled with one of the following carriers: Collagen, WTCP, thrombin. β-TCP complex, or sodiumalginate (control group); the other cap was filled with therespective carrier soaked with PRP (experimental group). After 8 weeks, undecalcified samples were sectioned and used for histological observations and measurements of the amount … More of osteoneogenesis. In addition, longitudinal observations of osteogenesis guided by the β-TCP PRP complex were carried out.The most suitable method for collecting PRP was found to be a double spin method (2400 rpm for 10 mm followed by 3600 rpm for 15 mm), yielding a platelet concentration factor of 13.5. With respect to any of the carriers tested, the amount of osteoneogenesis in the cap was increased in the experimental PRP(+) group compared to the control PRP(-) group. The amount of osteogenesis was greater in the B-TCP PRP complex pair than in any of the other carrier pairs. In the longitudinal study of osteogenesis using the β-TCP PRP complex, PRP was found to increase osteogenesis relatively early in the study period.Since this experimental study confirms the osteogenesis-promoting effects of the β-TCP ・ PRP complex without the combined use of thrombin, this suggests that the 13-TCP ・ PRP complex alone will promote osteogenesis when used in clinical treatments. In addition this method, which uses no animal-derived thrombin, could have additional benefits in a clinical setting, preventing infection by unknown viruses or the side effects of abnormal blood coagulation.In 4 or 8 weeks, there was much increase of bone the PRP/β -TCP complex and CCEF stimulus modelthan PRP/β -TCP complex. Precise-ization of the bone and new bone formation promotion effect of the CCEF stimulating method was accepted. Less

本研究的目的是比较评估富血小板血浆（PRP）与不同载体材料联合使用对成骨的促进作用。我们通过在兔顶骨上固定一个帽来创建一个封闭的空间，以便使用引导骨再生（GBR）方法研究成骨。将PRP与骨补偿载体一起填充空间以诱导成骨，并测量帽内的成骨量。首先，我们评估了收集PRP的各种方法。然后我们在每只兔子的顶骨表面附着一对聚丙烯帽。其中一帽填充下列载体之一：胶原蛋白、WTCP、凝血酶。β-TCP配合物，或海藻酸钠（对照组）；另一个瓶盖分别装上分别浸泡过PRP的载体（实验组）。8周后，将未钙化的标本切片，用于组织学观察和测量成骨量。此外，还对β-TCP PRP复合物引导下的成骨过程进行了纵向观察。最适合收集PRP的方法是双旋法（2400转/分钟，10毫米，然后3600转/分钟，15毫米），血小板浓度因子为13.5。对于测试的任何载体，实验PRP（+）组与对照PRP（-）组相比，帽中的成骨量增加。B-TCP PRP复合物对的成骨量大于其他任何载体对。在利用β-TCP PRP复合物进行成骨的纵向研究中，PRP在研究期间相对较早地发现了促进成骨的作用。由于本实验研究证实了β-TCP·PRP复合物在不联合使用凝血酶的情况下促进成骨的作用，这表明在临床治疗中单独使用13-TCP·PRP复合物将促进成骨。此外，这种方法不使用动物源性凝血酶，在临床环境中可能有额外的好处，可以预防未知病毒感染或异常凝血的副作用。在第4周和第8周，骨PRP/β -TCP复合物和CCEF刺激模型明显高于PRP/β -TCP复合物。CCEF刺激方法对骨的精确化和新骨形成的促进作用被接受。少