An immunohistochemical study of the pericacellous oral mucosa -Relationship between epithelial dysplasia and expression of p53 and ki-67 protein

对口腔黏膜鳞状细胞癌周围黏膜异常病变45例31例进行临床和病理研究，并对相关细胞的细胞核DNA进行定量分析。得到了以下结果：1)原发肿瘤大小等于或小于T2的病例最多（87.1%），而在组织病理学上，高分化型鳞状细胞癌伴轻度细胞异型最常见（87.1%）。2)癌周粘膜异常目视检查显示，以白血病型最常见（67.1%），其次为白血病与红细胞型混合（16.1%），疣状型（6.5%）。3)关于癌之间的位置关系和不正常粘膜、癌的病例(I型)在在场的范围内粘膜异常最常见(48.4%),其次是第二组(II型)两人 ... 彼此更多的反对(32.3%),第三组(III型)的两个分离(6.4%),和一个第四组类型II和III是混合(12.9%)。4)按组织病理学分类，无发育不良的粘膜异常占12.9%，轻度发育不良占51.6%，中度发育不良占19.4%，重度发育不良占16.1%。5)在DNA倍性方面，30.4%的肿瘤细胞为二倍体，69.6%的肿瘤细胞为非整倍体，而异常粘膜细胞的DNA倍性分别为54.2%和45.8%。6)一般来说，细胞核DNA含量变化超过4C的异常粘膜细胞的频率处于癌细胞和正常上皮细胞相应值之间的中点。然而，在四例二倍体模式和五例非整倍体模式中，表现出变异并超过4C的细胞频率低于在癌细胞中观察到的下限。7)不典型增生与相关细胞的DNA倍体模式无明显相关性。与非发育不良细胞相比，发育不良细胞的细胞核DNA含量变异和超过4C的细胞频率更高，但没有观察到显著差异。综上所述，口腔粘膜癌周围的异常粘膜可能是口腔粘膜癌产生的基础，而非整倍体或变异细胞频率较高且核DNA含量超过4C的粘膜，表明其具有很强的恶性转变潜力。少

Clinical and pathological studies were performed on 31 cases from 45 lesions with abnormal mucous membranes around squamous cell carcinoma of oral mucosa, and nuclear DNA in the relevant cells was quantified. The following results were obtained.1) Cases whose primary tumor was at a stage equal to or less than T2 in size were most frequent (87.1%), while on a histopathological findings, the squamous cell carcinoma of well differentiated type with mildly cellular atypia were commonest (87.1%).2) Visual inspection of abnormal mucous membranes around carcinoma reveals that leukopathic type were the most common (67.1%), followed by a mixture of leukopathic and erythromatous type (16.1%), and verrucous type (6.5%).3) Regarding the positional relationship between carcinomas and abnormal mucous membranes, the cases (type I) in which a carcinoma was present within the confines of the abnormal mucous membranes were most frequent (48.4%),followed by a second group (type II) in which the two were … More opposed to each other (32.3%) by a third group (type III) in which the two were separated each other (6.4%), and by a fourth group in which types II and III were intermingled (12.9%).4) When classified on a histopathological basis, the abnormal mucous membranes lacking dysplasia accounted for 12.9%, those with slight dysplasia, 51.6%, those with moderate dysplasia, 19.4%, ad those with severe dysplasia, 16.1%.5) Regarding the DNA ploidy patter, diploidy pattern was observed in 30.4% of the carcinoma cells studied d aneuploidy pattern was observed in 69.6% of the cases while the equivalents for abnormal mucous membranes cells were 54.2% and 45.8%, respectively.6) Generally speaking, the frequency of the abnormal mucous membranes cells with a variation and exceeding 4C with respect to nuclear DNA content is at a midpoint between the corresponding values for the carcinima cells and the normal epithelial cells. However, in the four cases with a diploidy pattern and in the five cases with an aneuploidy pattern, the frequency of cells exhibiting a variation and exceeding 4C was below the lower limit observed in carcinoma cells.7) No apparent correlation was observed between the presence of dysplasia and the DNA ploidy pattern of the cells involved. Dysplastic cells showed a higher frequency of cells with a variatio and exceeding 4C with respect to the nuclear DNA content than did non-dysplasia, no significant differences were observed.On the basis of the above it is likely that the abnormal mucous membranes around carcinoma of oral mucosa may act as a basis of development from which such tumors are generated, and that the mucus membranes which have a higher frequency of cells with an aneuploidy pattern or a variation and exceeding 4C with respect to nuclear DNA content, suggest a strong potentiality to change malignancy. Less