An immunohistochemical study of the pericacellous oral mucosa -Relationship between epithelial dysplasia and expression of p53 and ki-67 protein

口腔黏膜周围细胞的免疫组化研究-上皮发育不良与p53、ki-67蛋白表达的关系

• 批准号: 14571919
• 负责人: KAWAHARA Kenji
• 金额: $ 1.92万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571919/
• 关键词: squamous cell carcinoma of oral mucosa; the pericancellous oral mucosa; potentiality to change malignancy; p53 protein; ki-67 protein; An immunohistochemical study; 予後

Clinical and pathological studies were performed on 31 cases from 45 lesions with abnormal mucous membranes around squamous cell carcinoma of oral mucosa, and nuclear DNA in the relevant cells was quantified. The following results were obtained.1) Cases whose primary tumor was at a stage equal to or less than T2 in size were most frequent (87.1%), while on a histopathological findings, the squamous cell carcinoma of well differentiated type with mildly cellular atypia were commonest (87.1%).2) Visual inspection of abnormal mucous membranes around carcinoma reveals that leukopathic type were the most common (67.1%), followed by a mixture of leukopathic and erythromatous type (16.1%), and verrucous type (6.5%).3) Regarding the positional relationship between carcinomas and abnormal mucous membranes, the cases (type I) in which a carcinoma was present within the confines of the abnormal mucous membranes were most frequent (48.4%),followed by a second group (type II) in which the two were … More opposed to each other (32.3%) by a third group (type III) in which the two were separated each other (6.4%), and by a fourth group in which types II and III were intermingled (12.9%).4) When classified on a histopathological basis, the abnormal mucous membranes lacking dysplasia accounted for 12.9%, those with slight dysplasia, 51.6%, those with moderate dysplasia, 19.4%, ad those with severe dysplasia, 16.1%.5) Regarding the DNA ploidy patter, diploidy pattern was observed in 30.4% of the carcinoma cells studied d aneuploidy pattern was observed in 69.6% of the cases while the equivalents for abnormal mucous membranes cells were 54.2% and 45.8%, respectively.6) Generally speaking, the frequency of the abnormal mucous membranes cells with a variation and exceeding 4C with respect to nuclear DNA content is at a midpoint between the corresponding values for the carcinima cells and the normal epithelial cells. However, in the four cases with a diploidy pattern and in the five cases with an aneuploidy pattern, the frequency of cells exhibiting a variation and exceeding 4C was below the lower limit observed in carcinoma cells.7) No apparent correlation was observed between the presence of dysplasia and the DNA ploidy pattern of the cells involved. Dysplastic cells showed a higher frequency of cells with a variatio and exceeding 4C with respect to the nuclear DNA content than did non-dysplasia, no significant differences were observed.On the basis of the above it is likely that the abnormal mucous membranes around carcinoma of oral mucosa may act as a basis of development from which such tumors are generated, and that the mucus membranes which have a higher frequency of cells with an aneuploidy pattern or a variation and exceeding 4C with respect to nuclear DNA content, suggest a strong potentiality to change malignancy. Less

本文对45例口腔粘膜鳞癌周围粘膜病变中的31例进行了临床和病理研究，并对相关细胞核DNA进行了定量分析。结果表明：（1）原发肿瘤以T_2或T_2以下者最多见（87.1%），而在组织病理学结果，高分化型鳞状细胞癌伴轻度细胞浸润最常见（87.1%）。2）肉眼观察癌周粘膜异常，以白斑型最多见其次为白血病型和红细胞瘤型混合型疣状型占16.1%（6.5%）。3）关于癌与异常粘膜的位置关系，在异常粘膜范围内存在癌的病例（I型）最常见（48.4%），其次是第二组（II型），其中两组均为 ...更多信息 彼此相对（32.3%）第三组（第三类）两者相互分离（6.4%），以及第四组，其中II型和III型混合（12.9%）。4）按组织病理学分类，无异型增生的异常粘膜占12.9%，轻度异型增生占51.6%，中度异型增生占51.6%，19.4%，重度异型增生16.1%。5）DNA倍体类型：二倍体占30.4%，异倍体占69.6%，异常粘膜细胞分别占54.2%和45.8%。核DNA含量变化超过4C的异常粘膜细胞的频率位于癌细胞和正常上皮细胞的相应值之间的中点。然而，在具有二倍体模式的四个病例和具有非整倍体模式的五个病例中，表现出变异并超过4C的细胞的频率低于在癌细胞中观察到的下限。7）在异常增生的存在和所涉及的细胞的DNA倍性模式之间没有观察到明显的相关性。发育不良细胞核DNA含量变异和超过4C的细胞频率高于非发育不良细胞，但没有观察到显著差异。根据上述情况，口腔粘膜癌周围的异常粘膜可能是产生这种肿瘤的发展基础。并且具有较高频率的具有非整倍体模式或变异的细胞以及相对于核DNA含量超过4C的细胞的粘膜表明改变恶性的强潜力。少