Effect of stress on the progression of periodontitis

压力对牙周炎进展的影响

• 批准号: 14571984
• 负责人: GOTO Yasuharu
• 金额: $ 1.98万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571984/
• 关键词: stress; periodontitis; substance P; junctional epithelium; neutrophils; migration; endocytotic ability; rat; 歯周疾患

It has been suggested that, the link between inflammatory disease (such as periodontitis) and stress may lie in the release of neurotransmitters from sensory nerve fibers upon stimulation by external stimuli. Substance P(SP) has been implicated as one such neurotransmitter which may be associated with inflammation. The purpose of this study was to investigate the effect of SP on the progression of periodontitis. The effect of SP on junctional epithehal cells (JE cells) and neutrophils in the junctional epithelium (JE) was studied by light and electron microscopy using diaminobenzidine (DAB) reaction. Intravenous injection of SP (10μg/kg or 100μg/kg) or local application of SP at concentrations of 10^<-4> M for 15 min caused migration of neutrophils in the JE. Some of these migrating neutrophils released DAB-positive azurophil granules into the intercellular spaces of the JE cells. The JE cells at the coronal portion of the JE seemed to endocytose these granules and digest them in the cells. These findings suggest that SP released from nerve terminals in the JE enhances migration of neutrophils and endocytotic ability of neutrophils and JE cells to form a line of defense against harmful stimuli.

有人认为，炎症性疾病(如牙周炎)与应激之间的联系可能在于感觉神经纤维在受到外界刺激时释放神经递质。P物质(SP)被认为是一种与炎症有关的神经递质。本研究的目的是探讨SP在牙周炎进展中的作用。采用二氨基联胺(DAB)反应，用光镜和电子显微镜观察SP对交界上皮细胞(JE)和中性粒细胞的影响。静脉注射SP(10μg/kg或100μg/kg)或局部应用浓度为10~(-4)M的SP 15分钟可引起中性粒细胞在JE内迁移。其中一些迁移的中性粒细胞将DAB阳性的天青颗粒释放到JE细胞的细胞间隙中。位于乙脑冠部的乙脑细胞似乎内吞这些颗粒，并在细胞中消化它们。这些结果表明，JE内神经末梢释放的SP增强了中性粒细胞的迁移和中性粒细胞和JE细胞的内吞能力，形成了一道防御有害刺激的防线。