Basic study for development of a novel periodontal tissue regeneration therapy utilizing immobilized cell growth factors

利用固定化细胞生长因子开发新型牙周组织再生疗法的基础研究

• 批准号: 14571981
• 负责人: IKEZAWA Kazuhiko
• 金额: $ 1.92万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571981/
• 关键词: periodontal ligament cell; immobilized cell growth factors; periodontal tissue regeneration therapy; FGF-2; matricrine; ヘパリン親和性; パラクライン; ヒト歯根膜細胞; 固相化細胞増殖因子; FGFレセプター; ヘパリン新和性; 細胞内シグナル伝達機構

Matricrine effects of basic fibroblast growth factor (FGF-2) on periodontal ligament cells were my estigated using FGF-2 bound to immobilized heparin. In the presence of fetal bovine serum, FGF-2 bound to heparin increased proliferation of periodontal ligament cells and inhibited alkaline phosphatase activity and type I collagen production. Immobilized FGF-2 and FCS directly on tissue culture plates also up-regulated DNA synthesis and down-regulated alkaline phosphatase activity in periodontal ligament cells. These results indicated that immobilized growth factors such as FGF-2 and ECS could affect on periodontal ligament cells as well as those soluble growth factors. The accurate quantification of growth factor immobilized on tissue culture plates was impossible. Thus, it was hard to compare the effectiveness of each growth factor between immobilized form and soluble form in this study. However, in the case of application of growth factor for periodontal tissue regeneration, the utilization of, immobilized growth factor may take the place of slow delivery system of soluble growth factors. Further investigation will be needed in order to evaluate the effectiveness of immobilized growth factor s in periodontal tissues.

应用固定化肝素结合碱性成纤维细胞生长因子（FGF-2），观察苦参碱对牙周膜细胞的作用。在胎牛血清的存在下，FGF-2结合肝素增加牙周膜细胞的增殖，抑制碱性磷酸酶活性和I型胶原的产生。直接在组织培养板上固定FGF-2和FCS也上调牙周膜细胞的DNA合成和下调碱性磷酸酶活性。这些结果表明，固定化生长因子如FGF-2和ECS可以影响牙周膜细胞以及可溶性生长因子。固定在组织培养板上的生长因子的准确定量是不可能的。因此，在本研究中难以比较固定化形式和可溶性形式之间的每种生长因子的有效性。然而，在将生长因子应用于牙周组织再生的情况下，固定化生长因子的利用可代替可溶性生长因子的缓慢递送系统。为了评价固定化生长因子在牙周组织中的有效性，还需要进一步的研究。

项目成果

Kazuhiko Ikezawa, et al.: "Role of MAP kinase (ERKI and ERK2) in human periodontal ligament cells proliferation stimulated by FGF-2 and IGF-I"Japanese Journal of Conservative Dentistry. 46. 680-689 (2003)

Kazuhiko Ikezawa 等人：“MAP 激酶（ERKI 和 ERK2）在 FGF-2 和 IGF-I 刺激的人牙周膜细胞增殖中的作用”日本保守牙科杂志。

池澤一彦ら: "FGF-2とIGF-IによるMAPキナーゼ経路を介したヒト歯根膜由来細胞の増殖制御機構"日本歯科保存学雑誌. 46・5. 680-689 (2003)

Kazuhiko Ikezawa 等人：“FGF-2 和 IGF-I 通过 MAP 激酶途径控制人牙周膜来源细胞的增殖控制机制”日本保守牙科杂志 46・5（2003 年）。