Biological and genetic analysis of Vibrio vulnificus in the slime of tidal flats, seawater, and fishes colleted along Ariake Sea, Japan

日本有明海沿岸收集的滩涂、海水和鱼类的粘液中创伤弧菌的生物学和遗传分析

• 批准号: 14572194
• 负责人: SAGAWA Kimitaka
• 金额: $ 2.11万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14572194/
• 关键词: Vibrio vulnificus; Ariake Sea; hemolytic activity; hemolysis; Chikugo River; thiourea; pulsed-field gel electrophoresis; Colia nasus; 制限酵素; 遺伝子解析; 薬剤感受性

To investigate the seasonal change of Vibrio vulnificus in Ariake Sea, Japan, we attempted to isolate V.vulnificus from the slime of tidal flats, seawater, and fishes obtained from three harbors along Ariake Sea. The samples were collected twice a month from January to December,2001. Also, we determined the biological and genetic characteristics of the individual isolates.V.vulnificus were isolated throughout the year, but the isolation ratios were higher during the warmer season from June to October. The isolates in the warmer season were able to grow in culture media containing 0.5% NaCl, whereas those isolated in the remaining seasons could not. Moreover, the isolates in the warmer season showed greater hemolytic activity than those isolated in the remaining seasons. Those results suggest that V.vulnificus isolated in the warmer seasons are more vigorous in nature than those isolated in the remaining seasons.Genetic analysis by pulsed-field gel electrophoresis using 50microM thiourea was successfully achieved. However, no identical band pattern was detected among strains studied.

为了研究日本有明海创伤弧菌的季节性变化，我们试图从沿着有明海的三个港口的滩涂、海水和鱼类的粘液中分离创伤弧菌。2001年1月至12月每月采集两次样品。创伤弧菌全年均有分离，但在6 ~ 10月温暖季节分离率较高。在温暖的季节分离的菌株能够在含0.5%NaCl的培养基中生长，而在其余季节分离的菌株不能。此外，分离物在温暖的季节表现出更大的溶血活性比那些分离在其余的季节。这些结果表明，在温暖的季节分离的创伤弧菌比在其余季节分离的更旺盛的性质。然而，在所研究的菌株中没有检测到相同的带型。

项目成果

佐川公矯, 橋本好司, 他2名: "有明海におけるVibrio vulnificusの環境調査と細菌学的解析"Laboratory Clinical Practice. 20(2). 85-88 (2002)

Kimiyoshi Sakawa、Koji Hashimoto 等 2 人：“有明海创伤弧菌的环境调查和细菌学分析”实验室临床实践 20(2)。

Epidemiological and microbiological analysis of Vibrio Vulnificus collected along Ariake Sea, Japan

日本有明海沿岸采集的创伤弧菌的流行病学和微生物学分析

• 发表时间: 2002
• 期刊: Laboratory and Clinical Practice 20
• 作者: Kimitaka Sagawa;Kouji Hashimoto;Masaharu Kondo;Tetsuya Toyoda
• 通讯作者: Tetsuya Toyoda

有明海干潟汚泥、海水、魚介類中におけるVibrio vulnificusの季節別生息状況

有明海滩涂污泥、海水和海鲜中创伤弧菌的季节性栖息状况

• 发表时间: 2004
• 期刊: 臨床病理 52(7)
• 作者: 木下千恵;堀田吏乃;橋本好司;近藤正治;松枝智子;佐川公矯
• 通讯作者: 佐川公矯

Seasonal change of Vibrio vulnificus in the slime of tidal flats, seawater, and fishes collected along Ariake Sea, Japan.

日本有明海沿岸的滩涂粘液、海水和鱼类中创伤弧菌的季节变化。

• 发表时间: 2004
• 期刊: Rinsho Byori 52
• 作者: Chie Kinoshita;Rie Horita;Kouji Hashimoto;Masaharu Kondo;Tomoko Matsueda;Kimitaka Sagawa
• 通讯作者: Kimitaka Sagawa

有明海におけるVibrio vulnificusの環境調査と細菌学的解析

有明海创伤弧菌环境调查及细菌学分析

• 发表时间: 2002
• 期刊: Laboratory and Clinical Practice 20(2)
• 作者: 佐川公矯;橋本好司;近藤正治;豊田哲也
• 通讯作者: 豊田哲也