Immunoglobulin secretions in the MLN and IEL were enhanced in stressed rats

应激大鼠 MLN 和 IEL 中的免疫球蛋白分泌增强

• 批准号: 16500517
• 负责人: MINAMI Hisanori
• 金额: $ 2.43万
• 依托单位: Prefectural University of Kumamoto
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500517/
• 关键词: stress; immunoglobulin secretions; GALT; intestinal intraepithelial lymphocytes; mucosal immunity; 小腸上皮細胞間リンパ球

To study the effects of different types of stressors on immune function, the lymphocytes subsets and the immunoglobulin productions by the lymphocytes in stress exposed rats were determined. Physical (electric foot shock) or psychological (non-foot shock) stress was induced using the communication box. Stresses were exposed for 2h a day, and the treatments were maintained for 14 consecutive days. Lymphocytes were isolated from mesenteric lymph node (MLN) and spleen using lympholyte-rat, and intestinal intraepithelial lymphocytes (IEL) using percoll gradient.Foot shock stress altered the lymphocytes subsets, however, no significant changes in the lymphocytes subsets occurred in the psychological stress group.Foot shock stress increased in the immunoglobulin secretions in the MLN and Spleen lymphocytes, and also in IEL. Furthermore, psychological stress also caused increases in the IgA and IgG productions in the IEL. These results demonstrated the intestinal immune functions were regulated adaptively in moderate stress conditions.

为了研究不同应激源对大鼠免疫功能的影响，测定了应激大鼠淋巴细胞亚群和淋巴细胞免疫球蛋白的产生。利用通讯箱诱导物理（足电）或心理（非足电）应激。每天处理2h，连续处理14 d。采用淋巴细胞法分离大鼠肠系膜淋巴结（MLN）和脾脏淋巴细胞，采用percoll梯度法分离肠上皮内淋巴细胞（IEL）。足部休克应激改变了淋巴细胞亚群，而心理应激组淋巴细胞亚群无明显变化。足部休克应激增加了MLN和脾脏淋巴细胞的免疫球蛋白分泌，也增加了IEL。此外，心理应激也导致IEL中IgA和IgG的产生增加。这些结果表明，在中等应激条件下，肠道免疫功能受到适应性调节。

项目成果

ストレス負荷ラットにおける腸管リンパ球の抗体産生能亢進

应激大鼠肠道淋巴细胞抗体产生能力增加

• 发表时间: 2005
• 期刊: 消化と吸収 28・1
• 作者: 一橋 沙織;南 久則
• 通讯作者: 南 久則

An elemental diet controls inflammation in indonethacin-induced small bowel disease in rats : The role of low dietary fat and the elimination of dietary proteins

基本饮食控制大鼠吲哚美辛引起的小肠疾病的炎症：低膳食脂肪和消除膳食蛋白质的作用

• 发表时间: 2005
• 期刊: Digestive Diseases and Sciences 50
• 作者: Suzuki H;Hanyou N;Sonaka I;Minami H
• 通讯作者: Minami H

ストレス負荷ラットにおける腸管リンパ球の抗体産生亢進

应激大鼠肠道淋巴细胞抗体产生增加

• 发表时间: 2005
• 期刊: 消化と吸収 28,1
• 作者: 一橋沙織;南 久則
• 通讯作者: 南 久則

Immunoglobulin secretions in the mesenteric lymph node and intestinal lymphocytes in stressed rats

应激大鼠肠系膜淋巴结和肠淋巴细胞的免疫球蛋白分泌

• 发表时间: 2005
• 期刊: Digestion and Absorption 28,1
• 作者: Saori Ichihashi;Hisanori Minami
• 通讯作者: Hisanori Minami

An elemental diet controls inflammation inindomethacin-induced small bowel disease in rats : the role of low dietary fat and the elimination of dietary proteins

基本饮食控制大鼠吲哚美辛引起的小肠疾病的炎症：低膳食脂肪和消除膳食蛋白质的作用

• 发表时间: 2005
• 期刊: Digestive Diseases and Sciences 50(in press)
• 作者: H.Suzuki;N.Hanyou;I.Sonaka;H.Minami
• 通讯作者: H.Minami