Development of measurement of water content in articular cartilage using near infrared spectroscopy for diagnosis of osteoarthritis

使用近红外光谱测量关节软骨中的水含量以诊断骨关节炎的进展

• 批准号: 16560063
• 负责人: TANABE Yuji
• 金额: $ 2.37万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16560063/
• 关键词: Biomechanics; Articular cartilage; Osteoarthritis; Near infrared spectroscopy; Indentation test; Absorbance; Permeability; Mankin score; モンテカルロ法; 含水率; 検量線; Mankin Score

The objective of this study is to develop the method for the determination of mechanical properties of the degenerated articular cartilage through the measurement of water content. To this end, near infrared (NIR) spectroscopy which can measure water content quantitatively in terms of absorbance was used. Previous studies found differences in permeability between normal articular cartilage (hyaline cartilage), and OA (osteoarthritis) cartilage (fibrocartilage), and therefore different time-dependent water content after indentation testing was expected between these two cartilages.First, this study deals with the two aspects of the detailed relations between recovery of absorbance and degeneration of human cartilage, that is, 1) relation between the degeneration of human articular cartilage and pattern of absorbance with respect to time after removal of indentation load, 2) relation between the degeneration of human articular cartilage graded by Mankin Score and variation of absorbance … More with time after removal indentation load.Second, this study discusses the decreases of absorbance for cartilages and koya-tofu during compression. The recoveries of absorbance after indentation were classified into 3 patterns. Initial decrease and the subsequent increase of absorbance (pattern 1), immediate increase of absorbance after removal of indentation load (pattern 2), and no recovery of absorbance (pattern 3) were observed. In the most of specimens graded as "normal", the recovery pattern of absorbance was "pattern 1". In the specimens of "pattern 1", the Mankin Score was able to be related to the time when absorbance had the minimum value. In the specimens of "pattern 2", the mean ± S.D. of Mankin Score was 3.6±2.0. Cartilages showed the increase of absorbance during compression, while koya-tofu showed the decrease of absorbance during compression.It is concluded that NIR spectroscopy enables us to perform the low-invasive, nondestructive and quantitative measurement of water content in articular cartilage, and this can be applied to the diagnosis of the extent of osteoarthritis. Less

本研究的目的是开发通过测量水含量来测定退化关节软骨的机械性能的方法。为此，使用了近红外（NIR）光谱法，该光谱法可以根据吸光度定量测量水含量。先前的研究发现正常关节软骨（透明软骨）和OA（骨关节炎）软骨（纤维软骨）之间的渗透性存在差异，因此压痕测试后这两种软骨之间的时间依赖性含水量预计会有所不同。首先，本研究涉及吸光度恢复与人类软骨退化之间的详细关系的两个方面，即1） 人类关节软骨的退化和吸光度模式相对于去除压痕载荷后时间的关系，2）通过曼金评分分级的人类关节软骨的退化与去除压痕载荷后吸光度随时间的变化之间的关系。其次，本研究讨论了压缩过程中软骨和koya-tofu吸光度的降低。压痕后的吸光度回收率分为3种模式。观察到吸光度最初降低然后增加（模式 1），去除压痕载荷后吸光度立即增加（模式 2），并且吸光度没有恢复（模式 3）。在大多数分级为“正常”的样本中，吸光度的恢复模式为“模式1”。在“模式1”的样本中，Mankin评分能够与吸光度具有最小值的时间相关。在“模式2”的样本中，平均值±S.D. Mankin评分为3.6±2.0。软骨在受压过程中吸光度增加，而曲野豆腐在受压过程中吸光度降低。由此可见，近红外光谱技术使我们能够对关节软骨中的水分含量进行低侵入、无损、定量的测量，可应用于骨关节炎程度的诊断。较少的

项目成果

Development of low-invasive diagnosis for degenerated articular cartilage using near-infrared spectroscopy

利用近红外光谱技术开发退变关节软骨的低侵入诊断

• 发表时间: 2004
• 期刊: Proceedings of JSEM 2004 Annual Conference on Experimental Mechanics No.4-2
• 作者: Yuji Tanabe;Kazunari Hamada;Tatsuya Kikuchi;Makoto Sakamoto;Koichi Kobayashi;Go Omori;Yoshio Koga
• 通讯作者: Yoshio Koga

Measurement of water content in articular cartilage using near infrared spectroscopic meter -Fundamental examination about absorbance-

使用近红外光谱仪测量关节软骨中的水分含量-吸光度的基础检查-

• 发表时间: 2007
• 期刊: Proceedings of the 44th Annual Meeting of the JSME, 2007 Annual Meeting of Hokuriku-Shinetsu Vol.077-1
• 作者: Shinya Sato;Kazunari Hamada;Yuji Tanabe;Tatsuya Kikuchi;Koichi Kobayashi;Makoto Sakamoto;Yoshio Koga
• 通讯作者: Yoshio Koga

Measurement of water content in articular cartilage using near infrared spectroscopy (2nd report) -Calibration curve between NIR absorbance and water content-

使用近红外光谱法测量关节软骨中的水含量（第二次报告）-NIR吸光度和水含量之间的校准曲线-

• 发表时间: 2005
• 期刊: Proceedings of Mechanical Engineering Congress, 2005 Japan
• 作者: Kazunari Hamada;Yuji Tanabe;Tatsuya Kikuchi;Koichi Kobayashi;Makoto Sakamoto;Yoshio Koga
• 通讯作者: Yoshio Koga

近赤外線水分計を用いた軟骨含水量の測定 -吸光度に関する基礎的検討-

使用近红外线水分计测定软骨水分含量-吸光度的基础研究-

• 发表时间: 2007
• 期刊: 日本機械学会北陸信越支部 第44期総会・講演会 講演論文集
• 作者: 佐藤真也;濱田一成;菊池達哉;田邊裕治;小林公一;坂本信;古賀良生
• 通讯作者: 古賀良生

Quantitative evaluation of water content in articular cartilage using near infrared spectrometer

利用近红外光谱仪定量评价关节软骨中的含水量

• 发表时间: 2006
• 期刊: Japanese Journal of Clinical Biomechanics Vol.27
• 作者: Kazunari Hamada;Yuji Tanabe;Tatsuya Kikuchi;Koichi Kobayashi;Makoto Sakamoto;Yoshio Koga
• 通讯作者: Yoshio Koga