Study of immunomodulation on the intestinal immune responses by saccharides

糖类对肠道免疫反应的免疫调节研究

• 批准号: 16580108
• 负责人: HOSONO Akira
• 金额: $ 2.37万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580108/
• 关键词: polysaccharides derived from Bifidobacterium; IgA; Peyer's patch; mesenteric lymph node; lamina propria; cecal follicle; CD11c^+ cells; Bifidobacterium菌体多糖; パイエル板; IFN-γ; IL-6

Bifidobacterium immunomodulators (BIM) are composed of water-soluble polysaccharides and peptidoglycans, which are expected to modulate immune responses in the host. We demonstrated that oral administration of bacterial polysaccharides, BIM for 7 consecutive days induced the immunoresponses (e.g. cytokine production) on the murine lymphoid tissues (Peyer's patch ; PP, mesenteric lymphoid nodes ; MLN, and spleen ; SPL). This study is to clarify the distribution of BIM in the host gut-associated lymphoid tissues after single-shot administration of BIM orally, and we are investigating the immune responses of lamina propropria (LP) as an effector site for mucosal IgA secretion by oral administration. We observed the distribution of BIM in PP and LP after oral administration. We have shown that the BIM reached PP and small intestinal LP (S-LP) at the passed time of 1 hour after oral administration and the location of DIM distribution observed in PP tissue sections were almost as same as CD11c^+ cells. Then, BIM reached MLN at the passed time of 18 hours after oral administration. These results suggested that the BIM might pass through the villous M-cells directly and might be endcytosed and processed by CD11c^+ cell such as dendritic cells in PP. It was also suggested that the BIM might pass through the tight junctions of intestinal epithelial cells into LP directly, but not through the common mucosal immune system (CMIS), and then the BIM might be endcytosed by CD11^+ cell such as dendritic cell orally at the passed time of 1 hour after single-shot administration. The findings of the cytokines and IgA responses of LPL indicated that BIM might stimulate S-LPL to secrete IgA and cytokines by BIM feeding. In addition, It suggested that BIM could induce different immunoreactions of LPL in the intestinal location between small intestine and large intestine.

双歧杆菌免疫调节剂（BIM）由水溶性多糖和肽聚糖组成，预期可调节宿主的免疫应答。我们证明了连续7天口服细菌多糖BIM诱导小鼠淋巴组织（派伊尔集合淋巴结; PP，肠系膜淋巴结; MLN和脾脏; SPL）的免疫应答（例如细胞因子产生）。本研究旨在阐明口服单次注射BIM后BIM在宿主肠道相关淋巴组织中的分布，并研究口服给药后作为粘膜伊加分泌效应位点的固有层（LP）的免疫应答。观察BIM在PP和LP中的分布。我们已经证明，BIM在口服给药后1小时到达PP和小肠LP（S-LP），并且在PP组织切片中观察到的DIM分布位置与CD 11 c ^+细胞几乎相同。然后，BIM在口服给药后18小时到达MLN。这些结果表明，在PP中BIM可能直接穿过绒毛M细胞，并可能被CD 11 c ^+细胞如树突状细胞等内吞和加工。结果表明，BIM可能通过肠上皮细胞的紧密连接直接进入LP，而不通过共同粘膜免疫系统（CMIS）进入LP，并在单次给药后1h内被DC等CD 11 ^+细胞吞噬。LPL细胞因子和伊加反应的研究结果表明，BIM可能通过BIM饲养刺激S-LPL分泌伊加和细胞因子。此外，BIM在小肠和大肠之间的肠段可诱导不同的LPL免疫反应。

项目成果

Effects of sugar cane extract on the modulation of immunity in pigs.

甘蔗提取物对猪免疫调节的影响。

• 发表时间: 2005
• 期刊: The Journal of Veterinary Medical Science 67(6)
• 作者: D-Y.Lo;T-H.Chen;M-S.Chien;K.Koge;A.Hosono;S.Kaminogawa;W-C.Lee
• 通讯作者: W-C.Lee

III-3-1 腸管免疫細胞分離法 c)粘膜固有層リンパ球の調製法。(上野川修一・清水誠・八村敏志・戸塚護 編著) : 腸管細胞機能実験法

III-3-1肠道免疫细胞分离方法c)固有层淋巴细胞的制备方法。

• 发表时间: 2005
• 作者: A.Kikuchi;H.Nakajima-Adachi;A.Ebihara;Y.Takahashi;A.Hosono;K.Itoh;S.Hachimura;S.Kaminogawa;細野 朗
• 通讯作者: 細野 朗

Characteristic immune response in Peyer's patch cells induced by oral administration of Bifidobacterium components.

口服双歧杆菌成分诱导派尔氏斑细胞的特征性免疫反应。

• 发表时间: 2005
• 期刊: Cytotechnology 47
• 作者: Nakanishi Y;Hosono A;Hiramatsu Y;Kimura T;Nakamura R;Kaminogawa S
• 通讯作者: Kaminogawa S

III-3-1 腸管免疫細胞分離法 c)粘膜固有層リンパ球の調製法.:腸管細胞機能実験法(上野川修一・清水誠・八村敏志・戸塚護編著)

III-3-1 肠道免疫细胞分离方法 c) 固有层淋巴细胞制备方法：肠道细胞功能实验方法（上野川秀一、清水诚、八村敏志、户冢守编）

• 发表时间: 2005
• 作者: A.Kikuchi;H.Nakajima-Adachi;A.Ebihara;Y.Takahashi;A.Hosono;K.Itoh;S.Hachimura;S.Kaminogawa;細野 朗;細野朗
• 通讯作者: 細野朗

Establishment and analysis of germfree T cell receptor transgenic mice.

无菌T细胞受体转基因小鼠的建立与分析。

• 发表时间: 2004
• 期刊: Animal Cell Technology : Basic & Applied Aspects 13
• 作者: Fujioka M;Hachimura S;Hosono A;Nakamura R;Hirayama K;Itoh K;Kaminogawa S
• 通讯作者: Kaminogawa S