Studies on induction of multiple buds and adventitious embryo in Shorea spp.of Dipterocarpaceae

龙脑香科望天树多芽及不定胚诱导的研究

• 批准号: 16580129
• 负责人: KUBO Takafumi
• 金额: $ 2.3万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580129/
• 关键词: Multiple bud; Adventitious embryo; Diperocarpaceae; Shorea multiflora; Callus; Plant regulators; CO_2 exposure; MS medium; フタバガキ科樹種; 多芽体誘導; カルス誘導; 細胞形態; シュート化; 発根; 初期不定胚; 植物調整物質

1. Examination of culture conditions for inducing multiple buds from apical shout and lateral budIt was attempted to induce the multiple buds from apical shout of Shorea.multiflora by rotation culture in liquid medium, by relating to effects of various culture conditions, as MS medium and plant regulators, which were auxin of 2,4-D and cytokinin of BAP, zeatin and 4PU. Scale leaves were formed by exclusive application of BAP 3μM, but not by other conditions. The elongation was recognized by 25% of frequency, but these shouts died within a month. In this experiment, although multiple buds were not induced, it was suggested that cytokinin was effective for the formation of multiple buds.2. Examination of culture conditions for callus induction, evaluation of the differentiation potency and culture conditions for formation of early adventitious embryoInduction of callus with the differentiation potency was tried using leaves of S.multiflora, by relating to the effects of plant regulators … More of auxin (2,4-D) and cytokinin (BAP, 4PU, zeatin and TDZ), two kind of sugars (sucrose and maltose) and exposure of CO_2. The combination of 10μM of 2,4D and 3μM of BAP was the most effective for inducing frequently the excellent callus. Furthermore, the developed tissues like apical meristem or primary globular embryo was observed in the induced callus. Also, it was recognized that maltose, TDZ and exposure of CO_2 were useful for callus proliferation.3. Examination of culture conditions for shouting and rooting of the induced multiple budsIn this assignment, an effective result was not achieved for the study plan. As for rooting, it may need to examine in detail regarding to the modification of basic medium and the improvement of the concentration and combination of plant regulators in the future.4. Examination of culture conditions for maturation of primary embryoIt was attempted to maturate the tissue like a primary globular embryo, observed in the induction callus. Actually, the effects of Auxin and cytokinin were investigated to promote the maturation, but the development was not recognized in every culture conditions. Less

1.通过对不同培养条件（MS培养基、植物生长素2，4-D、细胞分裂素BAP、玉米素和4PU）的影响，对多花滨藜（Shorea multiflora）顶芽和侧芽诱导的培养条件进行了研究。单独施用BAP 3μM能形成鳞片叶，其它条件不能形成鳞片叶。伸长被25%的频率识别，但这些叫声在一个月内死亡。本实验中，虽然没有诱导出丛生芽，但细胞分裂素对丛生芽的形成有一定的作用.对愈伤组织诱导的培养条件、分化能力的评价和早期不定胚形成的培养条件进行了研究。 ...更多信息 生长素（2，4-D）、细胞分裂素（BAP、4PU、玉米素和TDZ）、蔗糖和麦芽糖以及CO_2暴露对植物生长的影响。10μM的2，4-D和3μM的BAP组合对频繁诱导优良的愈伤组织最有效。此外，在诱导的愈伤组织中观察到顶端分生组织或初生球形胚等发达组织。麦芽糖、TDZ和CO_2暴露有利于愈伤组织的增殖.诱导丛生芽生根培养条件的研究在本课题中，研究计划未取得有效结果。至于生根方面，今后可能需要对基本培养基的改良以及植物调节剂浓度和组合的改进进行详细的研究.初生胚成熟培养条件的研究在诱导愈伤组织中观察到的初生球形胚，试图使组织成熟。实际上，生长素和细胞分裂素的作用被研究以促进成熟，但并非在每种培养条件下都能识别发育。少