Studies on induction of multiple buds and adventitious embryo in Shorea spp.of Dipterocarpaceae

1. 尝试了香梨根尖茎和侧芽诱导多芽的培养条件研究。在液体培养基中轮作培养，根据不同培养条件的影响，作为MS培养基和植物调节剂，即2,4- d生长素和BAP、玉米素和4PU的细胞分裂素。单独施用BAP 3μM可形成鳞片状叶片，其他条件均未形成鳞片状叶片。延长的频率被识别为25%，但这些叫声在一个月内消失了。在本实验中，虽然没有诱导出多芽，但表明细胞分裂素对多芽的形成是有效的。研究了愈伤组织诱导的培养条件，评价了愈伤组织的分化能力和形成早期不定胚的培养条件。通过植物调节因子——生长素（2,4- d）和细胞分裂素（BAP、4PU、玉米素和TDZ）、两种糖（蔗糖和麦芽糖）和CO_2的作用，研究了愈伤组织的诱导能力。10μM的2、4D和3μM的BAP在诱导愈伤组织方面效果最好。在诱导愈伤组织中，可以观察到顶端分生组织或初生球形胚等组织的发育。麦芽糖、TDZ和CO_2对愈伤组织的增殖也有促进作用。对诱导多芽萌发生根的培养条件的考察在本课题中，研究计划没有取得有效的结果。在生根方面，今后还需要对基本培养基的改良、植物调节剂浓度和组合的提高等方面进行详细的研究。初生胚胎成熟培养条件的考察在诱导愈伤组织中观察到，试图使组织成熟为初生球形胚胎。虽然研究了生长素和细胞分裂素对黄芪成熟的促进作用，但并不是在所有的培养条件下都有促进作用。少

1. Examination of culture conditions for inducing multiple buds from apical shout and lateral budIt was attempted to induce the multiple buds from apical shout of Shorea.multiflora by rotation culture in liquid medium, by relating to effects of various culture conditions, as MS medium and plant regulators, which were auxin of 2,4-D and cytokinin of BAP, zeatin and 4PU. Scale leaves were formed by exclusive application of BAP 3μM, but not by other conditions. The elongation was recognized by 25% of frequency, but these shouts died within a month. In this experiment, although multiple buds were not induced, it was suggested that cytokinin was effective for the formation of multiple buds.2. Examination of culture conditions for callus induction, evaluation of the differentiation potency and culture conditions for formation of early adventitious embryoInduction of callus with the differentiation potency was tried using leaves of S.multiflora, by relating to the effects of plant regulators … More of auxin (2,4-D) and cytokinin (BAP, 4PU, zeatin and TDZ), two kind of sugars (sucrose and maltose) and exposure of CO_2. The combination of 10μM of 2,4D and 3μM of BAP was the most effective for inducing frequently the excellent callus. Furthermore, the developed tissues like apical meristem or primary globular embryo was observed in the induced callus. Also, it was recognized that maltose, TDZ and exposure of CO_2 were useful for callus proliferation.3. Examination of culture conditions for shouting and rooting of the induced multiple budsIn this assignment, an effective result was not achieved for the study plan. As for rooting, it may need to examine in detail regarding to the modification of basic medium and the improvement of the concentration and combination of plant regulators in the future.4. Examination of culture conditions for maturation of primary embryoIt was attempted to maturate the tissue like a primary globular embryo, observed in the induction callus. Actually, the effects of Auxin and cytokinin were investigated to promote the maturation, but the development was not recognized in every culture conditions. Less