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Genetic analysis of excitatory α_1-adrenergic receptor in insulin secreting islet β-cells of the rat

大鼠胰岛素分泌胰岛β细胞兴奋性α_1肾上腺素受体的遗传分析

基本信息

批准号：
16590161
负责人：
SUGA Sechiko
金额：
$ 1.86万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

In the insulin secretion of islet β-cells, the excitatory effect of norepinephrine (NE) linking to activation of phospholipase C (PLC) is known to be present, although that effect is masked by the inhibitory effect of NE through the pertussis toxin (PTX)-sensitive pathway. The present study was carried out to examined whether α_1-adrenoceptors were present in islet p-cells, and if present, what subtype of α_1-radrenoceptor was responsible for the excitatory action of NE.In the PCR analysis for islets, three bands of 212 bp,300 bp and 304 bp, corresponding α_<1A>-,α_<1B>- and α_<1D>-adrenoceptors, were obtained. Compared with 212 and 300 bands, 304 band was weak. These results indicate that in islets, there are three subtypes of α_1-adrenoceptors, i.e.,α_<1A>,α_<1B> and α_<1D>, and that α_<1A> and α_<1B> are more prominent than α_<1D>. The PCR analysis also showed the presence of such α_1-adrenoceptor subtypes in rat liver tissues. In order to explore the localization of these receptor subtypes, we examined immunofluorescence using receptor antibodies. In the islets of slice preparations of rat pancreas, none of α_<1A>-,α_<1B>- or α_<1D>-adrenoceptors were seen. In addition, these receptor subtypes were also not observed in the area out of the islets. Furthermore, from the immunofluorescence study on liver slices, these three subtypes of α_1-adrenoceptors were not observed. These results indicate that although the messages for α_1-adrenoceptor subtypes (α_<1A>,α_<1B>,α_<1D>) are present in rat islets, their locarization is not confirmed because of the use of inadequate antibodies for the receptors.

在胰岛β细胞的胰岛素分泌中，已知去甲肾上腺素(NE)与磷脂酶C(PLC)激活有关的兴奋作用，尽管这种作用被NE通过百日咳毒素敏感途径的抑制作用所掩盖。本研究旨在探讨胰岛P细胞中是否存在α_1-肾上腺素能受体，以及α_1-肾上腺素能受体的亚型与NE的兴奋作用有关。在胰岛的聚合酶链式反应分析中，获得了3条带，分别为212bP、300bP和304bP，分别对应于α_1、α_1B和α_1D&gt；与212和300条谱带相比，304条谱带较弱。这些结果表明，在胰岛中存在三种α_1受体亚型，即α_&lt；1A&gt；、α_&lt；1B&gt；和α_&lt；1d&gt；，且α_&lt；1A&gt；和α_&lt；1B&gt；比α_&lt；1d&gt；聚合酶链式反应分析还表明，在大鼠肝组织中存在α_1肾上腺素能受体亚型。为了探索这些受体亚型的定位，我们使用受体抗体检测了免疫荧光。在大鼠胰腺切片制备的胰岛中，未见α_1a、α_1b和α_1d&gt；受体。此外，在胰岛外区也未观察到这些受体亚型。此外，在肝切片的免疫荧光研究中，未观察到这三种α_1-肾上腺素能受体亚型。这些结果表明，虽然在大鼠胰岛中存在α_1-肾上腺素受体亚型(α_&lt；1A&gt；，α_&lt；1B&gt；，α_&lt；1d&gt；)的信息，但由于对受体的抗体不足，它们的定位尚未得到证实。

项目成果

期刊论文数量（14）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Staphylococcal enterotoxin A modulates intracellular Ca2+ signal pathway in human intestinal epithelial cells

DOI：
10.1016/j.febslet.2005.07.005
发表时间：
2005-08-15
期刊：
FEBS LETTERS
影响因子：
3.5
作者：
Hu, DL;Suga, S;Nakane, A
通讯作者：
Nakane, A

Pertussis toxin-sensitive pathway inhibits glucose-stimulated Ca2+ signals of rat islet beta-cells by affecting L-type Ca2+ channels and voltage-dependent K+ channels.

DOI：
10.1016/j.ceca.2004.04.006
发表时间：
2004-12
期刊：
Cell calcium
影响因子：
4
作者：
S. Suga;T. Takeo;Kyoko Nakano;Tatsusuke Sato;Takanori Igarashi;Daisuke Yamana;M. Wakui
通讯作者：
S. Suga;T. Takeo;Kyoko Nakano;Tatsusuke Sato;Takanori Igarashi;Daisuke Yamana;M. Wakui

Role of α7-nicotinic acetylcholine receptors in titanic stimulation-induced γossilations in rat hippocampal slices.

α7-烟碱乙酰胆碱受体在大鼠海马切片钛酸刺激诱导的 γ 振荡中的作用。