Investigation of beneficial effect of acupuncture treatment on wound healing in experimental model.

在实验模型中研究针灸治疗对伤口愈合的有益作用。

• 批准号: 16590557
• 负责人: OHNO Takamasa
• 金额: $ 1.22万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590557/
• 关键词: Postoperative sutura wound; Anti-inflammatory effect; Acupuncture treatment; Pathological investigation; Wound healing; Experimental animal model; 病理学的検計

In the present study, we investigated beneficial effect of acupuncture treatment on wound healing by pathological and biochemical methods in experimental model. It was hard to establish the mouse experimental model, because of their rapid natural healing.1. degree of severity in postoperative sutura wound :We estimated the degree of severity macroscopically. We can not find the effectiveness of acupuncture treatment in this model and methods.2. histology :The cell number of infiltrating cell to epidermides and corium were suppressed in acupuncture-treated group. The thickness of epidermides and corium were also suppressed in acupuncture-treated group.3. mRNA level in tissue (RT-PCR method) :The expression levels of inflammatory cytokine, TNF-a, IL-1b and IL-6, were suppressed in acupuncture-treated group.4. expression level of enzyme in tissue :There was no difference in expression level of tissue transglutaminase (75 kDa) two groups.5. serum TNF-α level :It increased very little.

在本研究中，我们通过病理和生化方法在实验模型中研究针刺治疗对创面愈合的有益作用。由于小鼠的自然愈合速度快，因此很难建立小鼠实验模型。术后缝合伤口的严重程度：我们从宏观上估计严重程度。在这种模式和方法中，我们没有发现针灸治疗的有效性。组织学：针刺组大鼠表皮和表皮浸润细胞数量均受到抑制。针刺组大鼠表皮层和表皮层厚度均受到抑制。组织mRNA水平（RT-PCR法）：针刺组炎性细胞因子TNF-a、IL-1b、IL-6的表达均受到抑制。组织酶表达水平：75 kDa组织转谷氨酰胺酶表达水平两组无差异。血清TNF-α水平：无明显升高。