Methodological establishment for vital observation of tumor microcirculation and development of cancer therapy based on the characteristics of tumor microvessl

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Lung metastasis is one of the worst factors for postoperative prognosis in primary lung cancer. In this study, we planed to develop new cancer therapy based on characteristics of tumor micro-vessel of lung metastasis through studies of vital observation of tumor microcirculation.During 2004, we established the method for making pulmonary metastasis of SATO lung cancer in Donryu rat, the system for vital observation of tumor microcirculation on surface of the lung by fluorescent microscopy, and the system for vital observation of tumor feeding arteries for lung metastasis by soft X ray micro-angiography.During 2005, we studied the characteristics of tumor micro-vessel by fluorescent microscopy and soft X ray micro-angiography.During 2006, we studied the effect of ZD6126 to tumor micro-vessel, the effect of chemo-immunotherapy (0K432 with CDDP) to anti-tumor lymphocytes, and the effect of hypoxia to feeding arteries of lung metastasis.(RESULTS)1) We established the model of lung metastasis of SATO lung cancer.2) We elucidated the characteristics of tumor micro-vessels.3) ZD6126 injure the tumor through the destruction of tumor micro-vessels.4) OK432 with CDDP accumulate anti-tumor lymphocytes to tumor micro-vessels.5) Hypoxia evokes vasoconstriction of tumor feeding arteries.This result indicates the ability of tumor vascular targeting therapy.