An experiment of making microaspiration pneumonia model mouse with applications of Micro-Fabrication technology

本文介绍了应用微加工技术制作小鼠微吸入性肺炎模型的实验研究结果。对8例与显微抽吸相关的尸检病例进行了显微镜分析。免疫组化染色显示肺泡1型上皮细胞和构成胸膜的梭形细胞凋亡阳性。在支气管上皮细胞和支气管周围平滑肌细胞中也发现了阳性结果。为了从实验病理学角度证实上述结果，对老年大鼠龋病模型组（实验组）和老年大鼠龋病模型组（对照组）进行了人工抽吸实验。与对照组相比，实验组大鼠肺组织中有大量嗜酸性细胞浸润，肺水肿明显。实验组支气管肺泡组织病理学改变为循环系统紊乱和纤维化。支气管上皮细胞抗ssDNA阳性率高，提示细胞凋亡加速引起脱落。支气管肺泡细胞凋亡加速与尸检结果相似。动物实验证实了衰老和慢性炎症导致的支气管上皮细胞凋亡加速，而对照组则表现出更多的衰老现象，而循环系统紊乱，支气管肺泡细胞脆性改变。慢性炎症引起的支气管上皮细胞脱落是吸入性肺炎发生的重要因素，而年龄变化如脆性改变则是重要因素。

This report is described about the results of the research titled "An experiment of making microaspiration pneumonia model mouse with applications of Micro-Fabrication technology". Eight autopsy cases that related microaspiration were analyzed microscopically. Positive findings of apoptosis were found in bronchoalveolar epithelial cells (type 1) and spindle cells which composing pleura, immunohistochemically. Positive findings were also found in bronchoepithelial cells and smooth muscle cells surrounding the bronchus。 This result suggested that acceleration of apoptosis found in these cells is one of important factor for microaspiration.For the purpose of confirmation for these findings using experimental pathology, artificial aspiration had done for senior rats group suffering caries (Experimental group) and senior rat group(Control group). Much infiltration of neutrocytes and pulmonary edema in experimental group were seen significantly compare to control group. In bronchoalveolar of experimental group, circulatory system disorder and fibrosis were found, pathologically. And much positive findings of anti ssDNA on bronchoepithelial cells was suggested that peeling off and/or falling off of cells caused by acceleration of apoptosiso This acceleration of apoptosis seen in bronchoalveolar cells was similar to the findings of autopsy cases. Acceleration of apoptosis seen in bronchoepithelial cells caused by aging and chronic inflammation was confirmed using animal experiment.On the contrary, there were much aging phenomenon were seen in control group compare to circulation system disorder; fragile change of bronchoalveolar cells. Falling off of bronchoepithelial cells caused by chronic inflammation was more important factor for aspiration pneumonia than aging change, such as fragile change.