An experiment of making microaspiration pneumonia model mouse with applications of Micro-Fabrication technology

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This report is described about the results of the research titled "An experiment of making microaspiration pneumonia model mouse with applications of Micro-Fabrication technology". Eight autopsy cases that related microaspiration were analyzed microscopically. Positive findings of apoptosis were found in bronchoalveolar epithelial cells (type 1) and spindle cells which composing pleura, immunohistochemically. Positive findings were also found in bronchoepithelial cells and smooth muscle cells surrounding the bronchus。 This result suggested that acceleration of apoptosis found in these cells is one of important factor for microaspiration.For the purpose of confirmation for these findings using experimental pathology, artificial aspiration had done for senior rats group suffering caries (Experimental group) and senior rat group(Control group). Much infiltration of neutrocytes and pulmonary edema in experimental group were seen significantly compare to control group. In bronchoalveolar of experimental group, circulatory system disorder and fibrosis were found, pathologically. And much positive findings of anti ssDNA on bronchoepithelial cells was suggested that peeling off and/or falling off of cells caused by acceleration of apoptosiso This acceleration of apoptosis seen in bronchoalveolar cells was similar to the findings of autopsy cases. Acceleration of apoptosis seen in bronchoepithelial cells caused by aging and chronic inflammation was confirmed using animal experiment.On the contrary, there were much aging phenomenon were seen in control group compare to circulation system disorder; fragile change of bronchoalveolar cells. Falling off of bronchoepithelial cells caused by chronic inflammation was more important factor for aspiration pneumonia than aging change, such as fragile change.