New bone formation using porous apatites with cultured bone marrow cells in back of rats

我们开发了多孔烧结碳酸盐磷灰石（CAP）块，并将其与从年轻成年大鼠获得的骨髓细胞相结合。这种杂交材料被植入大鼠的背部。骨髓细胞杂交是一种有用的骨诱导骨替代品。人工骨填充材料的应用已经得到了深入的研究。特别是对磷酸钙进行了研究。本研究的目的是比较四种骨填充材料对骨形成的影响。所采用的材料有：β-磷酸三钙、碳酸盐磷灰石、羟基磷灰石和聚苯乙烯作为对照。分别培养成年大鼠股骨骨髓基质细胞7天和14天。然后用Real Time RT-PCR法检测培养细胞mRNA的表达量。测定runx2、碱性磷酸酶、骨钙素、骨桥蛋白、骨粘连蛋白、骨涎蛋白、decorin、1型胶原蛋白的基因表达并进行统计学分析（Scheffe, p<0.05）。结果表明，β-TCP、CAP和HAP的mRNA表达量均高于聚苯乙烯（对照）。β -TCP、CAP和HAP三者间差异不显著。最后，将13 -TOP、CAP和HAP作为骨填充材料进行了应用，取得了较好的效果。然而，在骨形成方面，所研究的材料的容量并没有不同。

We have developed porous blocks of sintered carbonate apatite (CAP) and combined them with bone marrow cells obtained from young adult rats. This hybrid material was implanted into the back of rats. Within 4marrow-cell hybrid is useful as an osteoinductive bone substitute.the use of artificial bone filling materials has been deeply studied. In particular the Calcium phosphate has been investigated. The purpose of this study was to compare the effect of bone formation using four kinds of bone filling materials. The materials employed were, β-tricalcium phosphate, carbonate apatite, hydroxyapatite and polystyrene as control. Bone marrow stromal cells of femora from young adult rats were cultured for 7 and 14 days respectively. After that, the quantity of mRNA expression of the cells cultured was measured by Real Time RT-PCR method. The gene expression of runx2, alkaline posphatase, osteocalcin, osteopontin, osteonectin, bone sialoprotein, decorin and type1 collagen was measured and statistically analyzed (Scheffe, p<0.05). The results showed that the quantity of mRNA expression in β-TCP, CAP and HAP was higher than the polystyrene (control). However, there was no significant difference amongst β -TCP, CAP and HAP. Finally, the application of 13 -TOP, CAP and HAP as bone filling materials, demonstrated effectiveness. Nevertheless, concerning the bone formation, the capacities of the materials studied were not different.