Changes in the specificity of the interaction between cell growth/differentiation factors and glycosaminoglycans during development and differentiation.

发育和分化过程中细胞生长/分化因子与糖胺聚糖之间相互作用的特异性发生变化。

• 批准号: 17590078
• 负责人: YAMADA Shuhei
• 金额: $ 2.24万
• 依托单位: HOKKAIDO UNIVERSITY (2006)Kobe Pharmaceutical University (2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590078/
• 关键词: glycosaminoglycan; heparan sulfate; chondroitin sulfate; growth factor; model organism; Xenopus; Hydra; BlAcore

Glycosaminoglycans (GAGs) are complex sulfated polysaccharides with a backbone structure composed of a repeating disaccharide unit and occur in animal tissues. Highly heterogeneous sulfated patterns found in the GAG chains and the specific molecular interactions of GAGs with protein factors are involved in the regulation of various biological processes. To elucidate the relation between alterations in the fine structure and the changes in the specificity of the growth factor-binding during development, the following experiments were carried out. Furthermore, to better understand the structure-function relation of chondroitin sulfate (CS) chains, the highly sensitive method to determine CS oligosaccharide sequences at low pmol was also developed.1. Heparan sulfate and CS/dermatan sulfate chains were isolated from four different stages of Xenopus laevis embryos and their structure and growth factor-binding capacities were compared. Growth factor-binding experiments using a BlAcore system … More demonstrated that GAGs bound some growth factors with comparable affinities, which are, however, significantly varied during development. These results suggested that expression of saccharide sequences, which specifically interact with a growth factor, might be regulated during Xenopus embryogenesis.2. GAGs of Hydra magnipapillata were demonstrated and characterized. Immunostaining using anti-GAG antibodies showed that the epitope of anti-chondroitin antibody 473Al2 was localized in the developing nematocysts, which is a sting organelle specific to cnidarians. The chondroitin or CS proteoglycans might furnish an environment for assembling nematocyst components, and might be components of nematocysts.3. A variety of novel octa-and decasaccharides were isolated from shark cartilage CS-C as well as squid cartilage CS-E, and their saccharide sequences were determined using the newly developed sequencing method. These structurally-defined oligosaccharides were applied for characterization of the epitopes of several widely used anti-CS antibodies. Less

糖胺聚糖 (GAG) 是复杂的硫酸化多糖，其骨架结构由重复的二糖单元组成，存在于动物组织中。 GAG 链中发现的高度异质硫酸化模式以及 GAG 与蛋白质因子的特定分子相互作用涉及各种生物过程的调节。为了阐明发育过程中精细结构的改变和生长因子结合特异性的变化之间的关系，进行了以下实验。此外，为了更好地理解硫酸软骨素（CS）链的结构与功能关系，还开发了低pmol条件下测定CS寡糖序列的高灵敏度方法。 1．从非洲爪蟾胚胎的四个不同阶段中分离出硫酸乙酰肝素和CS/硫酸皮肤素链，并比较它们的结构和生长因子结合能力。使用 BlAcore 系统进行的生长因子结合实验表明，GAG 可以以相当的亲和力结合一些生长因子，然而，这些亲和力在发育过程中存在显着变化。这些结果表明，与生长因子特异性相互作用的糖序列的表达可能在非洲爪蟾胚胎发生过程中受到调节。2．对 Hydra magnipapillata 的 GAG 进行了论证和表征。使用抗GAG抗体的免疫染色表明，抗软骨素抗体473A12的表位位于发育中的线虫囊中，这是刺胞动物特有的刺细胞器。软骨素或CS蛋白聚糖可能为线虫囊成分的组装提供了环境，并且可能是线虫囊的组成部分。3．从鲨鱼软骨CS-C和鱿鱼软骨CS-E中分离出多种新型八糖和十糖，并使用新开发的测序方法测定了它们的糖序列。这些结构明确的寡糖用于表征几种广泛使用的抗 CS 抗体的表位。较少的

项目成果

Chondroitin sulfate characterized by the E-disaccharide unit is a potent inhibitor of herpes simplex virus infectivity and provides the virus binding sites on gro2C cells

• DOI: 10.1074/jbc.m503645200
• 发表时间: 2005-09-16
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Bergefall, K;Trybala, E;Bergström, T
• 通讯作者: Bergström, T

タンパク質の翻訳後修飾解析プロトコール

蛋白质翻译后修饰分析方案

• 发表时间: 2006
• 作者: Oohashi T;Bekku Y;Houliston RS;Van Sorge NM;Van Sorge NM;Overell J;Koga M;Kamitani T;Tatsumoto M;Nagasawa K;Houliston RS;Yoshida T;Funakoshi K;Tatsumoto M;Komagamine T;Gono T;Yuki N;Kimoto K;Comin R;Koga M;Koga M;Koga M;Pandey JP;Yuki N;Li J;Yuki N;Odaka M;Odaka M;Koga M;Hiraga A;Kuwabara S;Goodfellow JA;Takahashi M;Tatsumoto M;Kamitani T;S. Matsumiya;K. Kato;S. Matsumiya;K. Kato;Y.Wada;矢木宏和;S.Matsumiya;山口芳樹;C.-T.Guo;K.Kato;M.Holland;Y.Yamaguchi;K.Talabnin;Y.Kamiya;S.-J.Yoon;Y.Yamaguchi et al.;M.Holland et al.;Y.Yamaguchi et al.;Y. Kamiya;Y. Kamiya;T.Kimura et al.;H.Yagi et al.;Y.Kamiya et al.;S.Sekiya et al.;N.Takahashi;Y.Yamaguchi;Y. Yamaguchi;Y. Yamaguchi;Y.Yamaguchi;矢木宏和 他
• 通讯作者: 矢木宏和 他

Novel sulfated octa-and decasaccharides from squid cartilage chondroitin sulfate-E : Sequencing and application for determination of the epitope structure of monoclonal antibody MO-225.

来自鱿鱼软骨硫酸软骨素-E的新型硫酸化八糖和十糖：测序及其在单克隆抗体MO-225表位结构测定中的应用。

• 发表时间: 2007
• 期刊: Biochemistry 46 (9)
• 作者: Sarama Sathyaseelan Deepa;Kittiwan Kalayanamitra;Yumi Ito;Prachya Kongtaweelert;Shigeyuki Fukui;Shuhei Yamada;Tadahisa Mikami;Kazuyuki Sugahara
• 通讯作者: Kazuyuki Sugahara

Heparin-binding growth factor, pleiotrophin, mediates neuritogenic activity of embryonic pig brain-derived chondroitin sulfate/dermatan sulfate hybrid chains

• DOI: 10.1074/jbc.m413423200
• 发表时间: 2005-03-11
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Bao, XF;Mikami, T;Sugahara, K
• 通讯作者: Sugahara, K

Preface for the Special Issue Entitled "The research front line of the biosynthesis and recognition of acidic sugar chains".

《酸性糖链生物合成与识别的研究前沿》特刊序言。

• 发表时间: 2006
• 期刊: Trends in Glycoscience and Glycotechnology 18・101
• 作者: Tanaka;Y et al.;Shuhei Yamada
• 通讯作者: Shuhei Yamada