The investigation of regulatory mechanism on liver regeneration after massive hepatectomy
The investigation of regulatory mechanism on liver regeneration after massive hepatectomy
批准号:
17591408
负责人:
FUJII Masahiko
金额:
$2.3万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2005
资助国家:
日本
项目状态:
已结题
起止时间:
2005 至 2006
中文摘要
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英文摘要
Background)Liver failure after massive hepatectomy is one of the most important postoperative complication. Furthermore, in outbreak of liver failure after liver tranplantation, small-for-size graft is an important factor. In this study, we conducted to clarify the regulatory mechanism after massive hepatecomy and the control method for postoperative liver failure.Method and Results)1. Regulation of ischemic reperfusion injury (I/R injury) of the liver(1) follistatinIn rat model, improvement of transaminase levels and survival rate were not observed.(2)fluvastatinPreoperative treatment (oral administration) of fluvastatin decreased transaminase levels after I/R injury. Furthermore, we confirmed expression of eNOS, several cytokines and NOX4 on realtime PCR method after I/R injury.2. Gene expression after massive hepatectomy (90%)We analyzed gene expression after massive hepatectomy using the DNA microarray, and confirmed that Gene expression of STAT3 and HO-1 was enhanced after operati … More on.3. Effect of modulation on liver regeneration after massive hepatectomy(1)FK506In massive hepaatectomy (90%) model, FK506 accelerated liver regeneration in 72hr after operation. Furthermore, serum level of FK506 in massive hepatectomy (90%) model was higher than that in 70% hepatectomy model.(2)In-Chin-co-to (Japanese herbal medicine)Preoperative treatment (oral administration) of In-Chin-co-to which have effect of acceleration for biliary excretion improved postoperative survival and decreased transaminase levels. Furthermore, In-Chin-co-to accelerated liver regeneration after massive hepatectomy. In immunohisitochemical staining, expression of PCNA and HO-1 was significantly higher and a SMA was significantly lower.(3) fluvastatinPreoperative treatment (oral administration) of fluvastatin which have protection effect for I/R injury such as vascular statin accelerated liver regeneration and decreased total-billilubin level afar massive hepatectomy. In immunohisitochemical staining, expression of a SMA was suppressedSummary)In this study, we clarified the regulatory mechanism of liver regeneration and effect of several modulators for liver regeneration on massive hepatectomy using molecular biological method. However, we were not able to elucidate the regulation of liver regeneration using activin, because we had difficulty with the purification of activin. Less
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生体肝移植における過小グラフトの病態生理を治療戦略
活体肝移植中移植不足病理生理学的治疗策略
DOI:
--
发表时间:
2006
期刊:
四国医学会雑誌 62巻1,2号
影响因子:
--
作者:
[Young J, Barker MA, Simms LA, Walsh MD, Biden KG, Buchanan D, Buttenshaw R, Whitehall VL, Amold S, Jackson L, Kambara T, Spring KJ, Jenkins MA, Walker GJ, Hopper JL, Leggett BA, Jass JR, 島田 光生]
通讯作者:
島田 光生
The optimal administration of immunosuppressant for small-for-size graft in living doner living transplantation
活体移植中小型移植物免疫抑制剂的最佳给药方法
DOI:
--
发表时间:
2006
期刊:
Hepato-Gastroenterology 52
影响因子:
--
作者:
[Morine Y, Shimada M, Soejima Y, Fujii M, Imura S, Ikemoto T., Morine Y]
通讯作者:
Morine Y
特集/生体肝移植における門脈・肝静脈再建 II.門脈再建早期・晩期門脈血栓症
专题/活体肝移植中的门静脉和肝静脉重建II.早期和晚期门静脉血栓的门静脉重建。
DOI:
--
发表时间:
2006
期刊:
外科 68巻3号
影响因子:
--
作者:
[Takachi K, Doki Y, Ishikawa O, Miyashiro I, Sasaki Y, Ohigashi H, Murata K, Nakajima H, Hosoda H, Kangawa K, Sasakuma F, Imaoka S, Y.Morine, 森根 裕二]
通讯作者:
森根 裕二
Clincopathological features of uncontrorable recurrent hepatocellular carcinoma after non-surgical treatment
非手术治疗后无法控制的复发性肝细胞癌的临床病理特征
DOI:
--
发表时间:
2006
期刊:
Hepato-Gastroenterology Vol53, Supl.
影响因子:
--
作者:
[Young J, Barker MA, Simms LA, Walsh MD, Biden KG, Buchanan D, Buttenshaw R, Whitehall VL, Amold S, Jackson L, Kambara T, Spring KJ, Jenkins MA, Walker GJ, Hopper JL, Leggett BA, Jass JR, 島田 光生, Y.Morine, 森根 裕二, M.Kanamoto]
通讯作者:
M.Kanamoto
DOI:
--
发表时间:
2005
期刊:
Kan Tan Sui 50(1)
影响因子:
--
作者:
[Takachi K, Doki Y, Ishikawa O, Miyashiro I, Sasaki Y, Ohigashi H, Murata K, Nakajima H, Hosoda H, Kangawa K, Sasakuma F, Imaoka S, Y.Morine, 森根 裕二, M.Kanamoto, Morine Y]
通讯作者:
Morine Y
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