The investigation of regulatory mechanism on liver regeneration after massive hepatectomy

点击翻译按钮获取中文摘要

Background)Liver failure after massive hepatectomy is one of the most important postoperative complication. Furthermore, in outbreak of liver failure after liver tranplantation, small-for-size graft is an important factor. In this study, we conducted to clarify the regulatory mechanism after massive hepatecomy and the control method for postoperative liver failure.Method and Results)1. Regulation of ischemic reperfusion injury (I/R injury) of the liver(1) follistatinIn rat model, improvement of transaminase levels and survival rate were not observed.(2)fluvastatinPreoperative treatment (oral administration) of fluvastatin decreased transaminase levels after I/R injury. Furthermore, we confirmed expression of eNOS, several cytokines and NOX4 on realtime PCR method after I/R injury.2. Gene expression after massive hepatectomy (90%)We analyzed gene expression after massive hepatectomy using the DNA microarray, and confirmed that Gene expression of STAT3 and HO-1 was enhanced after operati … More on.3. Effect of modulation on liver regeneration after massive hepatectomy(1)FK506In massive hepaatectomy (90%) model, FK506 accelerated liver regeneration in 72hr after operation. Furthermore, serum level of FK506 in massive hepatectomy (90%) model was higher than that in 70% hepatectomy model.(2)In-Chin-co-to (Japanese herbal medicine)Preoperative treatment (oral administration) of In-Chin-co-to which have effect of acceleration for biliary excretion improved postoperative survival and decreased transaminase levels. Furthermore, In-Chin-co-to accelerated liver regeneration after massive hepatectomy. In immunohisitochemical staining, expression of PCNA and HO-1 was significantly higher and a SMA was significantly lower.(3) fluvastatinPreoperative treatment (oral administration) of fluvastatin which have protection effect for I/R injury such as vascular statin accelerated liver regeneration and decreased total-billilubin level afar massive hepatectomy. In immunohisitochemical staining, expression of a SMA was suppressedSummary)In this study, we clarified the regulatory mechanism of liver regeneration and effect of several modulators for liver regeneration on massive hepatectomy using molecular biological method. However, we were not able to elucidate the regulation of liver regeneration using activin, because we had difficulty with the purification of activin. Less