权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Estrogen enhances progesterone-induced stimulation of proliferation and differentiation of osteoprogenitors in cell populations derived from adult human bone

雌激素增强黄体酮诱导的成人骨细胞群中骨祖细胞增殖和分化的刺激

基本信息

批准号：
17591576
负责人：
ISHIDA Yoichiro
金额：
$ 1.86万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591576/
关键词：
osteoprogenitor progestin glucocorticoid estrogen

项目摘要

Previous experiments have demonstrated that bone cell populations derived from explants of lumbar vertebral bone of adult female rats contain osteoprogenitors that require dexamethasone (Dex) or progesterone (Prog) to proliferate and differentiate into fully differentiated bone-forming osteoblasts. This study was conducted to determine whether these findings found in rat are also observed in human using human cells. First, the present study was undertaken to determine the frequency and/or number of Dex and Prog-responsive osteoprogenitors in cell populations. Frequencies and numbers of progenitor types were determined for up to six subcultures using continuous subculturing, limiting dilution analysis, and colony assays. In Dex-containing medium, subculturing resulted in a 10% increase in the total number of Dex-responsive osteoprogenitors in second subculture cells over first subculture cell. From the third subculture onward, the frequency of osteoprogenitors decreased in a linear mann … More er and none were observed after five subcultures. Similar results were obtained in Prog-containing medium. Limiting dilution analysis in the presence of Dex indicated that 1.81% of cells represented a colony forming unit-fibroblast and 0.25% represented an osteoprogenitor in first subculture cells. In the presence of Prog indicated that 1.75% of cells represented a colony forming unit-fibroblast and 0.09% represented an osteoprogenitor in first subculture cells. Second, we show that the Prog-dependent population can be detected in female bone after sexual maturation, and can be induced by culturing the osteoprogenitors in media containing 17 β-estradiol (10-9-10-8m). This suggested that the survival of the Prog-dependent osteoprogenitors and/or their ability to proliferate is dependent on the presence of estrogen. The results presented here are compatible with the view that in the female skeleton one of the mechanisms maintaining bone mass is related to effects of both estrogen and Prog on a class of osteoprogenitors responsive to Prog. These findings may provide new insight into the significance of a specific class of Prog-dependent osteoprogenitors and of their regulation by estrogen in the human skeleton. Less

先前的实验已经证明，来自成年雌性大鼠腰椎骨外植体的骨细胞群含有骨祖细胞，其需要地塞米松（Dex）或孕酮（Prog）增殖并分化成完全分化的成骨细胞。本研究旨在确定在大鼠中发现的这些发现是否也在使用人类细胞的人类中观察到。首先，进行本研究以确定细胞群中Dex和Prog-responsive骨祖细胞的频率和/或数量。使用连续传代培养、有限稀释分析和集落测定法，确定了多达6次传代培养的祖细胞类型的频率和数量。在含Dex的培养基中，继代培养导致第二次继代培养细胞中Dex反应性骨祖细胞的总数比第一次继代培养细胞增加10%。从第三次传代开始，骨祖细胞的频率呈线性下降， ...更多信息在5次传代培养后未观察到ER。在含Prog-containing培养基中获得了类似的结果。在Dex存在下的有限稀释分析表明，在第一次传代细胞中，1.81%的细胞代表集落形成单位-成纤维细胞，0.25%代表骨祖细胞。在存在Prog的情况下，表明在第一次传代培养细胞中，1.75%的细胞代表集落形成单位-成纤维细胞，0.09%代表骨祖细胞。第二，我们发现在性成熟后的女性骨中可以检测到Prog-dependent群体，并且可以通过在含有17 β-雌二醇（10-9-10- 8 m）的培养基中培养骨祖细胞来诱导。这表明Prog-dependent骨祖细胞的存活和/或其增殖能力依赖于雌激素的存在。这里提出的结果是兼容的观点，在女性骨骼的机制之一，保持骨量有关的影响，雌激素和Prog对一类骨祖细胞响应Prog。这些发现可能提供了新的见解的意义，一类特定的Prog-dependent骨祖细胞和雌激素在人类骨骼中的调节。少