Effects of anti TNF α antibody (infliximab) plus MTX on SCID-HuRAg-pit model

抗TNFα抗体（英夫利昔单抗）联合MTX对SCID-HuRAg-pit模型的影响

• 批准号: 17591600
• 负责人: OCHIAI Akira
• 金额: $ 2.11万
• 依托单位: Toin University of Yokohama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591600/
• 关键词: Rheumatoid Arthritis; osteoclast; anti-TNF antibody

We already developed SCID-HuRAg-pit model which is dentin slice are engrafted with rheumatoid arthritis (RA) synovial tissues on back of SCID mice.In this study, we investigate effects of infliximab alone or infliximab plus methotrexate (MTX) for SCID-HuRAg-pit model. Administration of infliximab was using by osmotic pump. Methotrexate was administered orally 3 times per week. Control group was administrated human IgG. The engrafted synovial tissues were taken 4 weeks after transplantation and measured pit-formation. Moreover, plasma TNF-a, IL-6 were also measured. Plasma TNF-a and IL-6 concentrations were decreased in the group of infliximab administrated. However, there is no significant finding on the number of pit-formation on dentin. On the other hand, in case of infliximab plus MTX were significant differences about the number of pit-formation on dentin. These results suggest that the anti TNF-α antibody and MTX combination was useful against inhibition of bone destruction.

我们已经建立了SCID- hurag -pit模型，将牙本质切片植入SCID小鼠背部的类风湿关节炎滑膜组织。在这项研究中，我们研究了英夫利昔单抗单独或英夫利昔单抗加甲氨蝶呤（MTX）对SCID-HuRAg-pit模型的影响。英夫利昔单抗采用渗透泵给药。甲氨蝶呤每周口服3次。对照组给予人IgG。移植后4周取移植后的滑膜组织，测量窝形成情况。同时测定血浆TNF-a、IL-6。英夫利昔单抗组血浆TNF-a和IL-6浓度降低。然而，牙本质上凹坑形成的数量没有明显的发现。另一方面，英夫利昔单抗与MTX在牙本质上形成的坑数有显著差异。这些结果提示抗TNF-α抗体和MTX联合使用对骨破坏有抑制作用。

项目成果

抗カルパスタチン抗体(ACA)

抗钙蛋白酶抑制蛋白抗体 (ACA)

• 发表时间: 2005
• 期刊: 日本臨床-関節リウマチ 成因研究から治療の新時代へ- 63(9)
• 作者: 松野博明;落合晃;岩城(江川)祥子;渡辺泰裕
• 通讯作者: 渡辺泰裕