The contribution and modulation of vascular endothelial growth factor for brain edema formation

血管内皮生长因子对脑水肿形成的贡献和调节

• 批准号: 17591634
• 负责人: IIDA Yasuhiko
• 金额: $ 2.18万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591634/
• 关键词: Brain edema; Brain ischemia; Fat emboli; Vascular endothelial growth factor; Astrocyte; Basal lamina; 血管内皮増速因子

The purpose of the present study was to examine the temporal profiles of astrocytic responses, water channel aquaporin 4(AQP4) and vascular endothelial growth factor(VGEF) in the process of brain edema formation in the fat embolism model in rat, comparing to those in focal brain ischemia. Brain water increased, reaching almost maximum at 2 h after intracarotid injection of triolein. Increased AQP4 immunostaining were observed in the peri-affected area but was absent in the core of the lesion 2h after triolein injection, and reactive astrocytes with hypertrophy and mitosis were observed 72h after triolein injection. VGEF immunostaining was also increased in astrocyte 2hr after triolein injection and was more significant 24h and 72h after triolein injection.Basal lamina disappearance started to happen after 2h triolein injection and were more severe 24h and 72h after triolein injection. Neither significant increase of brain water content nor significant changes in AQP4 immunostaining were seen 2 h after MCAO. Our results indicate that AQP4, VGEF and basal lamina disappearance contribute to the brain edema formation of fat emboli. The modulation of these factors could lead the brain edema treatment.

本研究旨在探讨脂肪栓塞模型大鼠脑水肿形成过程中星形细胞反应、水通道水通道蛋白4（AQP4）和血管内皮生长因子（VGEF）的时间分布，并与局灶性脑缺血模型进行比较。脑水增加，在颈动脉内注射三油醇后2 h几乎达到最大。注射三油苷2h后，病变周围AQP4免疫染色升高，病灶核心AQP4免疫染色缺失；注射三油苷72h后，可见反应性星形细胞肥大、有丝分裂。星形胶质细胞的VGEF免疫染色在注射后2h也有所增加，在注射后24h和72h更为显著。注射三油苷2h后基底膜消失开始发生，注射后24h和72h基底膜消失更为严重。MCAO后2 h脑含水量未见明显增加，AQP4免疫染色未见明显变化。我们的结果表明，AQP4、VGEF和基底膜消失参与了脂肪栓塞脑水肿的形成。这些因素的调节有助于脑水肿的治疗。