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Relationship between central perceptivity of noradrenalin and oral dryness

去甲肾上腺素中枢感知能力与口腔干燥的关系

基本信息

批准号：
17591944
负责人：
HONDA Eiko
金额：
$ 2.24万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Activation of the subfornical organ (SFO) induces drinking behavior, that is, oral dryness. In this study, the effects of NA and its analogues on SFO neurons in rat slice preparations were investigated by using whole cell patch clamp recording. In the current clamp mode, the application of NA at 10-100 μM produced membrane depolarization (63%, 17 responsive neurons /27 neurons tested) and hyperpolarization (22%, 6/27 neurons). In the voltage clamp mode, NA application at 1-100 μM produced inward currents (69%, 42/61 neurons) and outward currents (23%, 14/61 neurons). These currents were remained in the presence of TTX or in the coapplication of glutamate and GABA receptor antagonists. In most of the neurons (25/31 neurons) showing inward currents in the presence of NA, the membrane conductance was not changed by voltage ramps or hyperpolarizing pulse stimulation. Similar responses were obtained by the application of the αl agonist phenylephrine. The phenylephrine-induced inward currents were inhibited by the α1 antagonist prazosin. In addition, the α2 agonist clonidine decreased the frequency of spontaneous GABAergic inhibitory postsynaptic currents (4/10 neurons). The results suggest that SFO neurons in rats are activated postsynaptically through α1 adrenoceptors, and that the activation is enhanced by suppressing GABAergic inhibitory synaptic inputs through presynaptic α2 adrenoceptors.

穹隆下器(SFO)的激活会导致饮酒行为，即口腔干燥。本研究采用全细胞膜片钳记录技术，观察NA及其类似物对大鼠脑片SFO神经元的影响。在电流钳模式下，10-100μM的NA可引起膜去极化(63%，17个反应神经元/27个神经元)和超极化(22%，6/27个神经元)。在电压钳模式下，在1~100μM的NA作用下，产生内向电流(69%，42/61个神经元)和外向电流(23%，14/61个神经元)。这些电流在TTX存在时或在谷氨酸和GABA受体拮抗剂共同应用时保持不变。在NA存在下呈现内向电流的大部分神经元(25/31个神经元)，膜电导不受电压斜波或超极化脉冲刺激的影响。应用αL激动剂苯肾上腺素也有类似的反应。α-1拮抗剂哌唑嗪可抑制苯肾上腺素诱发的内向电流。此外，α-2激动剂可乐定可降低自发性GABA能抑制性突触后电流的频率(4/10个神经元)。结果提示，大鼠孤束核神经元是通过α-1肾上腺素受体在突触后激活的，而通过突触前α-2受体抑制GABA能抑制性突触输入可增强这种激活。

项目成果

期刊论文数量（25）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Transient outward K+ currents in rat dissociated subfornical organ neurones and angiotensin II effects.

大鼠分离穹窿下器官神经元中的瞬时外向 K 电流和血管紧张素 II 效应。

DOI：
发表时间：
2005
期刊：
Journal of Physiology 568(3)
影响因子：
0
作者：
K.Ono;T.Toyono;E.Honda;K.Inenaga
通讯作者：
K.Inenaga

The functional evaluation of salivary glands using dynamic MR sialograph following citric acid stimulation : a preliminary study.

柠檬酸刺激后使用动态 MR 唾液记录仪对唾液腺进行功能评估：初步研究。

DOI：
发表时间：
2005
期刊：
Oral Surg Oral Med Oral Pathol Oral Radiol Endod 100
影响因子：
0
作者：
Morimoto Y;Ono K;Tanaka T;Kito S;Inoue H;Shinohara Y;Yokota M;Inenaga K;Ohba T.
通讯作者：
Ohba T.

Relationship of chewing-stimuIated whole saliva flow rate and salivary gland size

咀嚼刺激的全唾液流量与唾液腺大小的关系