Investigation of Comprehensive Vision of Transcriptional regulation in Bacillus subtilis

枯草芽孢杆菌转录调控的综合视野研究

• 批准号: 12206010
• 负责人: FUJITA Yasutaro
• 金额: $ 61.76万
• 依托单位: Fukuyama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12206010/
• 关键词: Bacillus subtilis; transcriptional regulation; network; DNA microarray; sigma factor; two-component regulatory system; HTH regulatory protein; regulon; HTH蛋白質

More than 500 Bacillus subtilis DNA microarray data were obtained, using the cells grown in various cultivation conditions and exposed to numerous environmental stresses, to perform gene clustering of altered genes, and using the transcriptional regulatory mutants, to extract the target gene candidates of the transcriptional regulatory factors. A fundamental network of metabolic regulation concerned with the biosynthesis of branched-chain amino acids involving carbon and nitrogen regulations by CcpA, CodY and TnrA and stringent control conducted by RelA, was revealed from the data of gene clustering and identification of target gene candidates.On the base of the DNA microarray data of DNA-binding transcriptional regulatory factors, we completed comprehensive molecular genetic analysis of ECF-sigma factors, and almost completed that of 34 two-component regulatory systems. Consequently, the function of only several two-component systems remained to be unknown. Moreover, we performed the molecular genetic analysis of the MarR, TetR and MerR families of HTH-DNA binding regulatory proteins among more than 200 proteins, for which the DNA maicroarray analysis of were almost completed, identifying at least one target gene of almost all proteins of the three families. Especially, it is notable that we found that YsiA protein was involved in global fatty acid degradation regulation. These results of the five-year project, unveiling a broad outline of the transcriptional network of B. subtilis, was published in 39 scientific papers listed below together with more than 100 of presentations at scientific meetings.

使用在各种培养条件下生长并暴露于多种环境胁迫的细胞，获得了500多个枯草芽孢杆菌DNA微阵列数据，以进行改变的基因的基因聚类，并使用转录调节突变体，提取转录调节因子的靶基因候选物。通过基因聚类和候选靶基因的鉴定，揭示了一个涉及支链氨基酸生物合成的基本代谢调控网络，包括CcpA、CodY和TnrA的碳、氮调控和RelA的严格调控。我们完成了ECF-sigma因子的全面分子遗传分析，并基本完成了34个双组分调控系统的分子遗传分析。因此，只有几个双组分系统的功能仍然是未知的。此外，我们还对200多个HTH-DNA结合调节蛋白中的马尔R、TetR和MerR家族进行了分子遗传学分析，并对这三个家族的几乎所有蛋白进行了基因芯片分析，确定了至少一个靶基因。特别值得注意的是，我们发现YsiA蛋白参与了全局脂肪酸降解调控。这些五年计划的结果，揭开了B转录网络的大致轮廓。枯草芽孢杆菌，发表在下面列出的39篇科学论文中，以及在科学会议上的100多个演讲。

项目成果

Recent progress in Bacillus subtilis two-component regulation.

• DOI: 10.2741/ogura
• 发表时间: 2002-08
• 期刊: Frontiers in bioscience : a journal and virtual library
• 作者: M. Ogura;Teruo Tanaka

Identification of two myo-inositol transporter genes of Bacillus subtilis

• DOI: 10.1128/jb.184.4.983-991.2002
• 发表时间: 2002-02-01
• 期刊: JOURNAL OF BACTERIOLOGY
• 影响因子: 3.2
• 作者: Yoshida, KI;Yamamoto, Y;Fujita, Y
• 通讯作者: Fujita, Y

Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons : Identification of its binding site and functional analysis of lmrB and yxaGH

枯草芽孢杆菌 LmrA 是 lmrAB 和 yxaGH 操纵子的阻遏物：鉴定其结合位点以及 lmrB 和 yxaGH 的功能分析

• 发表时间: 2004
• 期刊: Journal of Bacteriology 186 (17)
• 作者: Matsuoka;S.;Asai K;Sadaie;Y.;Satoshi Matsuoka;Tatsuya Fukushima;Masakuni Serizawa;Ken-ichi Yoshida
• 通讯作者: Ken-ichi Yoshida

Hideaki Nanamiya: "Involvement of ClpX protein in the post-transcriptional regulation of a competence specific transcription factor, ComK protein, of Bacillus subtilis"Journal of Biochemistry (Tokyo). 133(3). 295-302 (2003)

Hideaki Nanamiya：“ClpX 蛋白参与枯草芽孢杆菌能力特异性转录因子 ComK 蛋白的转录后调节”生物化学杂志（东京）。

Kazuo Kobayashi: "Essential Bacillus subtilis genes"Proceedings of National Acadmy of Science of the United States of America. 100(8). 4678-4683 (2003)

小林一夫：《枯草芽孢杆菌必需基因》美国国家科学院院刊。