Heart-specific antibodies as modulators of myocardial infarction (B06)

心脏特异性抗体作为心肌梗塞的调节剂 (B06)

• 批准号: 499097994
• 金额: --
• 依托单位: Deutsches Zentrum für Herzinsuffizienz Würzburg
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/499097994?language=en
• 关键词: Heart; specific; antibodies; modulators; myocardial

Antibodies are causally linked to the progression of ischemic HF. Yet, conventional serological approaches have thus far failed to identify what antigens are targeted and provide key mechanistic details regarding how antibodies impact myocardial biology. Herein, we will combine high-end imaging and state-of-the-art molecular biology techniques to track the specific antibody-producing B-cells activated after myocardial infarction and clone their antigen receptors (membrane-bound antibodies) into expression vectors. The subsequent monoclonal expression of the antibodies in mice and patients will ultimately allow in-depth functional characterization of their specifitieis for the first time.

抗体与缺血性心衰的进展有因果关系。然而，传统的血清学方法迄今未能确定哪些抗原是靶向的，并提供抗体如何影响心肌生物学的关键机制细节。在此，我们将结合高端成像技术和最先进的分子生物学技术来追踪心肌梗死后活化的特异性抗体产生b细胞，并将其抗原受体（膜结合抗体）克隆为表达载体。随后在小鼠和患者中进行抗体的单克隆表达，最终将首次对其特异性进行深入的功能表征。