Hybrid approach by electron and X-ray structural analysis for large macromolecular assemblies towards pseudo-atomic resolution

为了阐明各种生物功能背后的大型生物大分子复合物的机制，并将其用于医学和纳米技术的应用，必须以原子细节可视化其3D结构。电子低温显微镜（cryoEM）和大型复合物的图像分析，虽然很难实现原子分辨率本身，可以与组成分子的X射线晶体学相结合，以建立复合物的伪原子模型。我们提高了cryoEM图像分析的分辨率和通量，以显著提高复合物原子模型构建的准确性和效率，以促进我们对生物机制的理解。

To elucidate the mechanisms of large biological macromolecular complexes underlying various biological functions and to make use of them in the application for medicine and nanotechnology, it is essential to visualize their 3D structures in atomic detail. Electron cryomicroscopy (cryoEM) and image analysis of large complexes, although difficult to achieve atomic resolution by itself, can be combined with X-ray crystallography of component molecules to build pseudo-atomic models of the complexes. We have improved the resolution and throughput of cryoEM image analysis to increase the accuracy and efficiency of atomic model building of the complexes markedly to facilitate our understanding of the biological mechanisms.