Hybrid approach by electron and X-ray structural analysis for large macromolecular assemblies towards pseudo-atomic resolution

为了阐明生物大分子复合体的各种生物学功能的机制，并将其应用于医学和纳米技术，显示其原子细节的三维结构是必不可少的。电子冷冻显微镜和大型络合物的图像分析虽然很难单独实现原子分辨率，但可以与组分分子的X射线结晶学相结合来建立络合物的伪原子模型。我们提高了低温电子显微镜图像分析的分辨率和吞吐量，显着提高了络合物原子建模的准确性和效率，有助于我们对生物机制的理解。

To elucidate the mechanisms of large biological macromolecular complexes underlying various biological functions and to make use of them in the application for medicine and nanotechnology, it is essential to visualize their 3D structures in atomic detail. Electron cryomicroscopy (cryoEM) and image analysis of large complexes, although difficult to achieve atomic resolution by itself, can be combined with X-ray crystallography of component molecules to build pseudo-atomic models of the complexes. We have improved the resolution and throughput of cryoEM image analysis to increase the accuracy and efficiency of atomic model building of the complexes markedly to facilitate our understanding of the biological mechanisms.