High efficient production of secondary metabolites on the basis of genomes of Streptomyces

基于链霉菌基因组的次级代谢产物的高效生产

基本信息

批准号：
17019009
负责人：
池田治生
金额：
$ 83.46万
依托单位：
Kitasato University (2009)The University of Tokyo (2005-2008)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2009
项目状态：
已结题

项目摘要

The elucidation of the reaction mechanism of the various second metabolisms, which were observed in actinomycete microorganisms, was systematically examined. The results obtained in this study were applied to the improvement in pathway engineering, metabolic engineering, combinatorial biosynthesis and the creation of the artificial biosynthetic gene cluster for the production of new metabolites. Furthermore, the technology developed in this study was applied to improve the production level of not only known products but also unnatural products. Results would be useful for the development of new bioactive compounds in medical field and industrial materials. For the above purpose, we investigated and evaluated the detailed biosynthetic pathways and regulatory system(s) for their expression from the genome data of avermectin-producing Streptomyces avermitilis, Nocardia farcinia and streptomycin-producing Streptomyces griseus. We have also clarified the reaction mechanism of some biosynthesis gene clusters and novel reactions were identified (Baeyer-Villiger oxidation and new type of benzene ring formation). We also focused on cytochrome P450, flavonoid, and type-III polyketide synthesis and new unnatural flavonoids were discovered by above technology.

在放线菌微生物中观察到的各种第二种代谢的反应机理的阐明。在这项研究中获得的结果应用于途径工程，代谢工程，联合生物合成以及人工生物合成基因簇的改进，以生产新代谢产物。此外，这项研究中开发的技术不仅提高了已知产品的生产水平，而且还提高了不自然产品的生产水平。结果对于开发医疗领域和工业材料中新的生物活性化合物将很有用。出于上述目的，我们研究并评估了详细的生物合成途径和调节系统的表达，从同位基因组产生的链霉菌链霉菌链霉菌，牛ac和链霉素产生的链霉菌链霉菌的基因组数据中。我们还阐明了一些生物合成基因簇的反应机理，并鉴定了新的反应（Baeyer-Villiger氧化和新型的苯环形成）。我们还专注于细胞色素P450，类黄酮和III型聚酮化合物合成，并通过上述技术发现了新的不自然类黄酮。

项目成果

期刊论文数量（112）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Genome mining in Streptomyces avermitilis: A biochemical Baeyer-Villiger reaction and discovery of a new branch of the pentalenolactone family tree.

DOI：
10.1021/bi900766w
发表时间：
2009-07-14
期刊：
BIOCHEMISTRY
影响因子：
2.9
作者：
Jiang, Jiaoyang;Tetzlaff, Charles N.;Takamatsu, Satoshi;Iwatsuki, Masato;Komatsu, Mamoru;Ikeda, Haruo;Cane, David E.
通讯作者：
Cane, David E.

A novel pair of terminal protein and telomere-associated protein for replication of the linear chromosome of Streptomyces griseus.

一对新型末端蛋白和端粒相关蛋白，用于复制灰色链霉菌的线性染色体。

DOI：
发表时间：
2008
期刊：
Biosci. Biotechnol. Biochem 72
影响因子：
0
作者：
Suzuki;H.;Marushima;K.;Ohnishi;Y.;Horinouchi;S.
通讯作者：
S.

Pentalenolactone biosynthesis; molecular cloning and assignment of biochemical function to Pt1H, a non-heme iron dioxygenase of Streptomyces avermitilis.

五烯内酯生物合成；

DOI：
发表时间：
2006
期刊：
J. Am. Chem. Soc. 128
影响因子：
0
作者：
Katsuyama;Y.;Matsuzawa;M.;Funa;N.;Horinouchi;S.;Masanori Funabashi;Setsu Hirano;Akimasa Miyanaga;Yasuo Ohnishi;大西康夫;Nobutaka Funa;Tatsuichiro Higashi;Sueharu Horinouch;Sueharu Horinouch;Jun-ya Kato;Yohei Katsuyama;Yohei Katsuyama;Yohei Katsuyama;Yohei Katsuyama;Hiromi Nishida;Hirokazu Suzuki;Hirokazu Suzuki;Akiko Tanaka;勝山陽平;稲村典昭;Z. You;Z. You;J.Kato;D.E.Cane;K.Chiba;Nobutaka Funa;Ikuo Miyahisa;Hirokazu Suzuki;Ayami Tomono;S.Hirano;C.N.Tetzlaff;Z.You
通讯作者：
Z.You

放線菌Streptomyces griseusにおいてA-ファクターに依存する新規菌体外蛋白質の検索

在灰色链霉菌中寻找依赖于 A 因子的新型胞外蛋白