Mechanism for regulation of DECODE complexes in gene activation

多种转录因子通过形成转录因子-DNA复合物来调节转录。细胞信号传导引起转录因子的化学修饰，其调节转录活性。此外，在TFs-DNA复合物的背景下，转录因子的化学修饰对其活性的影响，然而，在很大程度上是未知的。在这个坚固的，我们表明，磷酸化的转录因子Ets 1的“差异”调制Ets 1含有TFs-DNA复合物。此外，我们还通过对CBFβ对Runx 1的变构调节的NMR分析和对TCRα基因增强子上形成的由Ets 1、Runx 1和CBFβ组成的多蛋白-DNA复合物的晶体学分析，阐明了选择性调节含Ets 1的TFs-DNA复合物的潜在机制。

Multiple transcription factors (TFs) regulate transcription by forming TFs-DNA complexes. A cell signaling causes chemical modification of transcription factors, which modulates transcriptional activities. Besides, effect of chemical modification of transcription factors on their activity in the context of TFs-DNA complexes, however, is largely unknown. In this sturdy, we showed that the phosphorylation of a transcription factor Ets1 "differentially" modulates Ets1-containing TFs-DNA complexes. In addition, we clarified the underlying mechanism by which the selective modulation of Ets1-containing TFs-DNA complexes is achieved using NMR analyses of allosteric regulation of Runx1 by CBFβ and crystallographic analyses of multiprotein-DNA complex comprised of Ets1, Runx1 and CBFβ formed on the TCRα gene enhancer.